Name: Nest PETG Erlenmeyer Flasks with Transfer Cap
Brand: Nest Scientific
SKU: NEST-EFLASK-PETG-ASSY
Availability: InStock

<h2 dir="ltr"> What are PETG Erlenmeyer Flasks?</h2>
 PETG (polyethylene terephthalate glycol) Erlenmeyer flasks are single-use, sterile, BPA-free conical shake flasks manufactured from glycol-modified PET for suspension cell culture, microbial fermentation, and media preparation on orbital shakers. PETG offers optical clarity comparable to PC, a working temperature range of &minus;80 &deg;C to +60 &deg;C, superior impact resistance at low temperatures versus standard PET, and a BPA-free composition &mdash; advantages over PC in single-use workflows where autoclaving is not required, and BPA-sensitive cell culture applications are involved.&nbsp; PETG Erlenmeyer flasks are the correct choice for high-throughput single-use suspension culture, BPA-free applications, and labs that want to eliminate autoclave and washing steps in their shake flask workflow.
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<h2 dir="ltr">What you will find:</h2>
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125mL PETG Erlenmeyer Flasks for Screening Studies: Perfect for small-scale media optimization and high-speed orbital shaking.
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250mL PETG Erlenmeyer Flasks for Routine Expansion: Provides a stable environment for consistent cell growth and passage maintenance.
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500mL PETG Erlenmeyer Flasks for Batch Production: Optimized for increasing cell densities while maintaining superior gas exchange.
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1000mL PETG Erlenmeyer Flasks for Large-Scale Recovery: Engineered for high-volume bioprocessing and bulk reagent preparation.
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<h2 dir="ltr"> How to Choose PETG Erlenmeyer Flasks</h2>
 PETG vs. PC for Shake Flask Culture PETG flasks are single-use and cannot be autoclaved (maximum temperature +60 &deg;C). PC flasks can be autoclaved 5&ndash;10 times and have a broader temperature range (&minus;135 &deg;C to +135 &deg;C). For high-throughput suspension culture operations processing many flasks per week, single-use PETG eliminates all washing, autoclave queuing, and glassware inspection steps &mdash; reducing hands-on time and contamination risk from reuse. For labs with lower throughput that prepare media in-house via autoclave, PC remains the cost-effective choice. PETG is BPA-free; PC contains BPA. Cell Line Compatibility PETG Erlenmeyer flasks are validated for CHO-S, HEK293-F, Expi293, Jurkat, K562, Sf9 (Spodoptera frugiperda), Hi5 (Trichoplusia ni), S. cerevisiae, P. pastoris, and E. coli suspension culture. The non-treated PETG surface prevents inadvertent cell adhesion and is compatible with all standard suspension culture media, including FreeStyle CHO, Expi293, Sf-900, and ESF 921 insect cell media. PETG is chemically resistant to DMSO, ethanol, isopropanol, and standard aqueous buffers. Baffled vs. Flat Base for PETG Flasks Baffled-base PETG Erlenmeyer flasks are the standard for oxygen-demanding suspension cultures: CHO, HEK293-F, bacterial, and insect cell lines. The baffles generate turbulent flow during orbital shaking, raising dissolved oxygen and reducing cell sedimentation. Flat-base PETG flasks are used for media preparation, gentle mixing, and shear-sensitive cell lines where turbulent conditions cause cell damage. NEST Scientific offers baffled and flat-base versions in both 125 mL and 1,000 mL. Vented Cap Gas Exchange NEST Scientific PETG Erlenmeyer flasks ship with a 0.22 &micro;m hydrophobic PTFE membrane vented cap in an HDPE housing with an oversized vent area. For suspension culture with 20&ndash;25% fill volume, vented caps at standard orbital speeds (100&ndash;150 RPM) provide adequate oxygen mass transfer to support mammalian cell densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting higher densities or with very high specific oxygen uptake rates (sOUR), confirm dissolved oxygen by inline monitoring before scaling further in PETG flasks. Storage of Unused Sterile Flasks Store unused NEST Scientific PETG Erlenmeyer flasks at room temperature (15&ndash;30 &deg;C) in the original sealed sterile bag, away from UV light. Shelf life is typically 3 years from the production date per the lot certificate. Inspect the bag seal before use; a compromised seal invalidates sterility. Remove from the bag inside a biosafety cabinet immediately before inoculation. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PETG Erlenmeyer flasks are manufactured from USP Class VI medical-grade PETG in ISO 8 cleanrooms. E-beam sterilized to SAL 10⁻⁶; each lot certified DNase/RNase-free, pyrogen-free, non-cytotoxic. Temperature range: &minus;80 &deg;C to +60 &deg;C. HDPE cap with 0.22 &micro;m PTFE hydrophobic membrane; oversized vent area for improved gas transfer. NEST Scientific is ISO 9001:2015 and ISO 13485 certified; FDA registered. NEST Scientific PETG Erlenmeyer flasks in 125 mL, 250 mL, 500 mL, and 1,000 mL with baffled and flat-base options are stocked by MBP. 
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PETG Erlenmeyer Flasks

Erlenmeyer Flasks

Key Specifications

Nest PETG Erlenmeyer Flasks with Transfer Cap

NEST PETG Erlenmeyer Flasks with Transfer Cap have a bi-directional cap, heat-seal TPE tubing and 0.22 µm vent filter for closed suspension culture, in 500 and 1000 mL, sterile, from MBP.