One of the vital innovations in the field of biomedical science is the invention of DNA ladders. In this blog, you’ll get a comprehensive insight into different types of DNA Ladders, including the most widely used 1KB Plus DNA ladders.
DNA Ladder Defined
Molecular-weight size markers or DNA ladder can be defined as a solution composed of DNA molecules of different lengths utilized in gel electrophoresis methods such as acrylamide gel electrophoresis or agarose. Typically, the DNA ladder is used to evaluate the size of DNA fragments that are unidentified by comparing their mobility in an electrical field utilizing the gel.
What Are They Used For?
DNA ladders permit researchers to differentiate and recognize different DNA fragments depending on their molecular weight, correlating with their size. DNA ladders are crucial in various DNA-related processes, like delineating between DNA variants and detecting the number of mutations in a DNA fragment.
Types Of DNA Ladder
Different types of DNA ladders differ in their functionality. These are:
- Standard DNA ladders−ranging from 100 base pairs (bp) to 10 kilobases (kb)
- High-resolution DNA ladders− ranging from 10 bp to 1 kb.
- Denatured DNA ladders− consist of DNA fragments that have been separated into individual strands.
- Quantitative DNA ladders− contain DNA fragments with known concentrations
- Low Molecular Weight ladders− consist of DNA fragments that are all shorter than 1,000 bp
- High Molecular Weight ladders− consist of DNA fragments that are all longer than 1,000 bp
1 KB Plus DNA Ladders Explained
This one is a DNA size standard widely used in molecular biology research to determine the size of double-stranded DNA fragments within the range of 150 base pairs (bp) to 25,000 bp. This DNA ladder is composed of DNA fragments ranging in length from 0.5 kilobases (kb) to 10 kb.
The 1KB Plus DNA ladders comprise fourteen individual DNA fragments, purified employing chromatography techniques. The purification procedure certifies the production of clear and sharp bands amid electrophoresis.
The 1KB Plus DNA ladders are meticulously manufactured, promising the accurate amount of DNA or number of DNA fragments in the ladder, permitting precise size estimation and unwavering results. These ladders can be utilized in agarose gels as well as polyacrylamide gels, with concentrations stretching from 0.7% to 1%.
Significance of 1KB Plus DNA Ladders
The 1KB Plus DNA ladders are prominently used in molecular biology research, typically used for the following applications:
Size determination− identifying the double-stranded DNA fragments’ size within the 250 to 25000 bp range.
Quantification− simplifies DNA fragment quantification by providing a standard for comparison.
Size range extension− allows researchers to determine the size of DNA fragments, larger than the conventional range covered by 1 KB ladders.
Quality control− serves as a quality regulator during the electrophoresis process.
Standardization− facilitates the standardization across various experiments and labs, enabling researchers to reproduce experiments, compare their results, and share their findings effectively.
The Key Takeaway
In short, DNA ladders are a widely used standard in molecular biology to determine the size of unidentified DNA fragments within a broad range. 1 KB Plus DNA ladder, in particular, is made of sharp bands, precise compatibility, and composition with numerous gel types, serving as one of the most invaluable assets in molecular biology experiments as well as DNA fragment analysis.
Find The Premium Quality 1 KB Plus DNA Ladders at MBP Inc.
If you want 1 KB Plus DNA ladders, contact MBP Inc. for accurate and efficient DNA size determination. At MBP Inc., we offer this comprehensive and versatile tool for your unparalleled convenience in producing reliable results regarding DNA analysis workflows.
Whether you are conducting PCR, gel electrophoresis, or DNA sequencing, our 1 KB Plus DNA ladders act as a catalyst in your laboratory toolkit for optimal results. Request a quote to know the price of our 1 KB Plus DNA ladder.