Name: Immobilized Pepsin Agarose Resin
Brand: Molecular Depot
SKU: B2015720
Price: 795.00 USD
Availability: InStock

<h2>What are activated agarose resins?</h2>
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Activated agarose resins are uncoupled agarose supports that have been chemically modified with a reactive group, such as NHS-activated, CNBr-activated, or hydrazide chemistry, so a researcher can covalently couple their own antibody, enzyme, or other ligand to build a custom affinity purification column. This differs from the pre-conjugated Protein A or Protein G agarose beads covered in the sibling Agarose Beads &amp; Resins category, which arrive ready to bind antibody without any coupling step.
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<h2 dir="ltr">What you will find: </h2>
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High-Porosity Structural Slurries: Non-spherical or bulk physical matrices engineered to support stable packing densities and excellent chemical resistance during manual separation steps.
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Low-Background Separation Backbones: Clean, unreactive polymer bases prepared to reduce background sticking and ensure maximum purity of the running target volume.
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Custom-Configurable Packing Media: Unloaded matrix configurations optimized to accept specialized chemical links or target-specific affinity ligands easily.
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<h2>How to choose an activated agarose resin</h2>
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Match the coupling chemistry to your ligand's functional groups
NHS-activated and CNBr-activated agarose both couple through primary amine groups on the ligand, making them suitable for most antibodies and proteins, while hydrazide resin couples through oxidized carbohydrate groups, which makes it well suited to glycosylated ligands like polyclonal antibodies where preserving the binding site orientation matters.
Decide between NHS and CNBr activation chemistry
NHS-activated agarose couples in an amine-free buffer at pH 7 to 9 with typical coupling efficiency above 80% regardless of the ligand's isoelectric point or molecular weight, while CNBr-activated agarose offers multi-point attachment that can produce a more chemically stable resin, though some studies report higher protein recovery yields with CNBr over NHS for specific antibodies.
Consider hydrazide resin for polyclonal antibodies
Coupling through the hydrazide group targets oxidized sugar groups concentrated in the antibody's Fc domain, which orients the antigen-binding site away from the resin and can reduce steric hindrance compared with amine-based coupling that may occur near the binding site.
Confirm whether a spacer arm matters for your ligand size
CNBr-activated agarose lacks a spacer arm and is generally preferred for large proteins and antibodies, while NHS-activated agarose includes a multi-atom spacer arm that can improve accessibility for smaller ligands or peptides.
Plan for the coupling reaction and recovery yield evaluation
Coupling efficiency is typically confirmed by measuring the ligand concentration remaining in the post-coupling filtrate via a protein assay, and comparing recovery yield between coupling chemistries for your specific antibody can reveal a meaningfully better-performing resin.
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<h2>Specifications context</h2>
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NHS-activated agarose coupling reactions are performed in an amine-free buffer at pH 7 to 9 with coupling efficiency typically exceeding 80% regardless of the ligand's isoelectric point or molecular weight, while CNBr-activated agarose has shown up to 50% higher protein recovery yield than NHS-activated agarose for some specific antibody-antigen systems in published comparisons. As of 2026, both NHS and CNBr activation chemistries remain standard choices for custom antibody coupling, with the better-performing option varying by the specific antibody and target protein involved.
<a href="https://mbpinc.net/contact-us">Contact the MBP technical support team</a> today to select the ideal resin grade for your internal column-packing needs.

Agarose Resins

Molecular Depot

Key Specifications

Immobilized Pepsin Agarose Resin

Immobilized Pepsin Agarose Resin by Molecular Depot is a agarose resin carrying immobilised pepsin. Pepsin cleaves immunoglobulin G below the inter-heavy-chain disulfides, which leaves the two binding arms joined as an F(ab')2 fragment and digests the Fc region into small pieces. It is used to prepare F(ab')2 fragments from immunoglobulin G, in antibody fragmentation and in controlled proteolysis. Each unit contains 1 mL. MBP supplies Molecular Depot immunoassay reagents to research labs in the United States, Canada and worldwide.