Name: Human EGF R Protein, Tag Free
Brand: Molecular Depot
SKU: B2024392
Price: 795.00 USD
Availability: InStock

<h2>What are epidermal growth factors?</h2>
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Epidermal growth factor (EGF) and heparin-binding EGF (HB-EGF) are recombinant signaling proteins that bind the EGF receptor (EGFR/ErbB1) to drive epithelial cell proliferation and survival. Related entities include organoid culture media, intestinal stem cell (Lgr5+) maintenance, keratinocyte culture, EGFR signaling pathways, and mammary epithelial cell lines.
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<h2>What you'll find in this category:</h2>
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Recombinant Human EGF: High-purity proteins optimized for stem cell maintenance and primary cell expansion.
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Tissue Regeneration Reagents: Stable growth factors designed for wound-healing assays and tissue culture applications.
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Mitogenic Signaling Tools: Recombinant proteins that support consistent and reproducible cell proliferation studies.
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<h2>How to choose EGF products</h2>
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Recombinant source and expression system
EGF produced in E. coli is the most common and cost-effective option for standard epithelial cell culture supplementation. Mammalian-expressed EGF can matter for glycosylation-sensitive applications, though human EGF is a small, non-glycosylated peptide in its native form, so E. coli-derived recombinant EGF is widely used.
Carrier-free vs. carrier-added formulation
Carrier-free EGF (no BSA) is preferred for animal-component-free (ACF) organoid and stem cell media where bovine protein could affect downstream single-cell or proteomic analysis. Carrier-added EGF offers improved long-term stability for routine epithelial culture supplementation.
Concentration and working range
Most organoid and epithelial culture protocols use EGF at 20&ndash;50 ng/mL in complete media. Confirm the working concentration specified in the published protocol before scaling an order, since under- or over-supplementation can affect proliferation rates and experimental consistency.
HB-EGF vs. standard EGF
HB-EGF binds heparan sulfate proteoglycans in addition to EGFR and is specified in some organoid and wound-healing protocols where matrix-bound signaling gradients are important. Standard recombinant EGF remains the default choice for most general epithelial maintenance media.
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<h2>Specifications context</h2>
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EGF activity is generally reported as ED50 in an EGFR-dependent proliferation or phosphorylation assay, alongside purity by SDS-PAGE (commonly &gt;95&ndash;98%). Pack sizes for academic laboratories typically range from 10 micrograms to 1 milligram, with bulk pricing tiers available for facilities running continuous organoid culture. As of 2026, organoid biobanking protocols increasingly specify carrier-free, low-endotoxin EGF to minimize lot-to-lot variability during long-term passaging.
This category sits alongside Interferons &amp; Interleukins and Fibroblast Growth Factors (FGF) within the broader Growth Factors &amp; Hormones catalog. Laboratories combining EGF with TGF-beta inhibitors for organoid differentiation may also review Transforming Growth Factors.
<a href="https://mbpinc.net/contact-us">Connect with the MBP sales desk</a> today to secure your essential EGF supplies for cell culture and organoid workflows.

Epidermal Growth Factors (EGF)

Molecular Depot

Key Specifications

Human EGF R Protein, Tag Free

Human EGF R Protein, Tag Free (B2024392) from Molecular Depot is the extracellular domain of human EGFR, supplied without a tag. Use Human EGF R protein, Tag free tag as the immobilized or soluble antigen in SPR and BLI kinetics, ELISA, antibody and nanobody screening, and receptor-ligand binding assays. Available from MBP, a registered vendor at leading US research institutions.