The precision and accuracy of even the best-calibrated pipette might be lost if the wrong type of tip is used. Depending on the experiment, the wrong kind of tip might also make your pipette a source of contamination, resulting in the waste of valuable samples or reagents.
There are numerous types of suggestions to pick from. How can you know which one is right for your pipette and situation? This brief blog will assist you in understanding your options so that you can select the proper tip, just like the Hamilton pipette tip, and avoid costly experimental errors.
Choose High-Quality Pipette Tips for Accuracy
When deciding which tip type to use, precision and accuracy are usually the first things that come to mind.
If the shape of the pipette tips varies from batch to batch or within a set, your pipetting will be inaccurate. This is a build quality and quality control issue, and it costs money, just like any other manufacturing process. So, avoiding cheap tips and investing in high-quality tips is often a better method to ensure that tip variability is kept to a minimum.
If the tip does not correctly fit your pipette, the accuracy of your pipette will suffer. If the pipette barrel and tip do not have a good seal, the drawn-in air can escape, and the correct volume of liquid is not inhaled. As a result, the ultimate volume discharged is not precisely accurate. It can be challenging to select a tip that is a good fit for your pipette.
Universal Tips
There is always the option of using the tips sold by the pipette maker if they are available. However, using high-quality universal tips is often the best solution for your pipette and application.
These universal tips are compatible with the majority of micropipettes on the market. Universal tips are made to fit securely and firmly around all pipette barrels, varying in diameter from one manufacturer to the next. Not all universal tips are created equal, so you must carefully consider your options.
Companies specializing in universal tip design have thoroughly investigated the problems that can occur with pipette tips and created technology to address them.
Filtered Or Non-Filtered Pipette Tips
Non-barrier and barrier tips, often known as filter tips, are intended for specific applications, like Hamilton filter tips. Non-barrier tips are usually employed for general laboratory use. However, suppose you plan to pipette something that could contaminate your pipette, such as volatile, acidic, or viscous compounds. In that case, you should consider filter tips to safeguard your pipette and your samples.
Aerosol Barrier Pipette Tips
Aerosol Barrier tips, also known as filter pipette tips, have a filter inside the proximal portion of the tip. Only a few high-end tips similar to Hamilton filter tips offer an excellent sealing barrier.
Due to the presence of barriers in these tips, they are ideal for delicate applications such as qPCR. The barrier avoids PCR contamination by preventing sample spillover from the pipette, resulting in more reliable findings.
Furthermore, filter tips serve as excellent ‘training wheels’ for newcomers. Pipette contamination frequently occurs when a new lab member unintentionally takes the liquid into the pipette itself.
Low-Retention Tips
Low retention is a significant feature regardless of the tip you use. Low-retention tips perform precisely what their name implies: they hold only a small amount of liquid. If you’ve ever glanced at a typical pipet tip, you may have seen a small amount of liquid remaining after dispensing. Low-retention tips prevent this by containing a hydrophobic plastic additive that prevents liquid from adhering to the inside of the tips.
Final Words
Your pipette and tip must serve together to produce accurate and exact measurements. Using this guide will assist you in selecting the optimum tip for each application, resulting in cleaner, more potent results.
We hope you like the above blog; contact Molecular Biology Products for the purchase of the best pipette tips. The pipette tips available on our website are authentic and can be used for all sorts of experiments. We have various pipette tips, including filtered, non-filtered, stackable Hamilton, Biomek i5 filter tips, and many more.
Citation:
(Biotix)
