Name: Hoagland's No. 2 Basal Salt Mixture without Ammonium Phosphate
Brand: Plant Cell Technologies
SKU: PCT-HOAGLAND-2-NOAP
Availability: InStock

<h2 data-section-id="10e7fg8" data-start="0" data-end="52">What types of tissue culture media are available?</h2>
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Tissue culture media for plant propagation fall into stage-specific categories built on a common nutrient foundation. Basal media, most commonly Murashige and Skoog (MS) formulation, supply the core macronutrients, micronutrients, and vitamins needed for general plant cell metabolism and serve as the starting point for nearly every protocol stage. Multiplication media build on a basal formulation with added cytokinin-dominant hormone ratios specifically formulated to drive the shoot proliferation stage of micropropagation. Related entities include macro/micronutrient salt composition, organic supplements (vitamins, sucrose, agar), the auxin-to-cytokinin developmental ratio, and subculture cycle timing.
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<h2 dir="ltr">What you will find: </h2>
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<a href="https://mbpinc.net/reagents/cell-biology/tissue-culture/plant-tissue-culture/tissue-culture-media/basal-media">Basal Media:</a> Globally trusted core nutrient formulations that provide the foundational salt mixtures and essential vitamins needed to sustain general plant cell viability and establish clean initial cultures.
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<a href="https://mbpinc.net/reagents/cell-biology/tissue-culture/plant-tissue-culture/tissue-culture-media/multiplication-media">Multiplication Media:</a> Specialized chemical blends optimized with specific hormone balances to stimulate axillary branching, drive rapid shoot proliferation, and maximize high-volume micropropagation yields.
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<a href="https://mbpinc.net/reagents/cell-biology/tissue-culture/plant-tissue-culture/tissue-culture-media/specialty-media">Specialty Media:</a> Tailored, species-specific nutrient environments engineered to satisfy the unique, demanding physiological requirements of rare, recalcitrant, or woody plant varieties.
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<a href="https://mbpinc.net/reagents/cell-biology/tissue-culture/plant-tissue-culture/tissue-culture-media/rooting-media" title="Rooting Media:">Rooting Media:</a> Nutrient-lean, auxin-supplemented media configurations designed to trigger robust adventitious root development and prepare delicate plantlets for successful soil acclimatization.
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<h2 data-section-id="i4dhjq" data-start="967" data-end="1004">How to choose tissue culture media</h2>
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Starting from basal medium vs. a pre-formulated stage-specific medium
Basal medium alone (such as plain MS salts) gives researchers full control to add their own growth regulators and supplements for any protocol stage, while pre-formulated multiplication media already include the appropriate cytokinin-rich hormone supplementation, reducing setup time and pipetting steps for routine, established protocols.
Powder vs. liquid concentrate vs. ready-to-use formats
Basal and multiplication media are available as powders requiring reconstitution and pH adjustment, liquid concentrates for dilution, or ready-to-use liquid media. Powders offer the lowest shipping cost and longest shelf life, while ready-to-use formats save preparation time for labs prioritizing throughput over cost.
Vitamin and organic supplement inclusion
Some basal medium formulations include only the inorganic salt mixture, requiring separate addition of vitamins and other organics, while complete formulations bundle salts, vitamins, and sometimes sucrose together; confirm which components are included before assuming a specific published protocol's full formulation is covered by a single product.
Matching hormone concentration to the multiplication stage's published protocol
Pre-formulated multiplication media are designed around documented cytokinin-to-auxin ratios optimized for shoot proliferation, but the exact concentration can be species- and genotype-dependent; confirm a chosen multiplication medium's hormone concentration against published precedent for the specific target plant before scaling up.
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<h2 data-section-id="1fljvas" data-start="3335" data-end="3360">Specifications context</h2>
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Basal media are reconstituted at defined concentrations, commonly around 4.3&ndash;4.4 g/L for standard MS formulations, with final pH typically adjusted to approximately 5.7&ndash;5.8 before autoclaving. Multiplication media specify cytokinin concentration (commonly BAP or kinetin in the low micromolar to mg/L range) alongside low or no auxin, reflecting the high cytokinin-to-auxin ratio that defines the shoot proliferation stage.&nbsp;
This sub-category sits within MBP's plant tissue culture catalog and contains basal media and multiplication media. MBP's specialist team can help confirm media format and hormone concentration for a specific species and micropropagation stage before order placement.
<a href="https://mbpinc.net/contact-us">Inquire with the MBP support team</a> today to order the ideal basal or specialized media blend for your facility.

Tissue Culture Media

Plant Cell Technologies

Basal Media

Hoagland's No. 2 Basal Salt Mixture without Ammonium Phosphate

Hoagland's No. 2 Basal Salt Mixture without Ammonium Phosphate (HOP02) by Plant Cell Technology is an ammonium-free Hoagland formulation for nitrogen metabolism and nutrient-form research, supplied in 10 L and 50 L packs. Variant catalog numbers: HOP02-10LT (10 L), HOP02-10LT.1 (10 L, alternate packaging), and HOP02-50LT (50 L). Available from MBP, a registered vendor at leading US research institutions.