Name: Ferrous Sulphate Heptahydrate for Cell Culture
Brand: Molecular Depot
SKU: B2016033
Price: 595.00 USD
Availability: InStock

<h2>What are research inorganic salts?</h2>
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Research inorganic salts are ionic compounds formed from a metal cation and an inorganic anion, used throughout molecular biology and biochemistry as buffer components, ionic strength agents, enzyme cofactor sources, and media supplements. The most frequently used are sodium chloride (NaCl) and potassium chloride (KCl) for ionic strength control; magnesium chloride (MgCl2) and magnesium sulfate (MgSO4) as essential cofactors for DNA polymerases, restriction enzymes, and kinases; calcium chloride (CaCl2) for calcium-dependent enzymes and cell permeabilization; and dibasic and monobasic phosphate salts for PBS and other phosphate-buffered systems.
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<h2 dir="ltr">What you will find:</h2>
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High-purity preservation agents formulated to prevent microbial growth and extend the shelf life of stored antibodies and liquid reagents.
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Premium salt crystals calibrated for regulating osmotic pressures and establishing baseline ionic profiles in delicate biochemical assays.
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<h2>How to choose inorganic salts</h2>
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Use magnesium chloride or magnesium sulfate as enzyme cofactors for polymerases and kinases
Mg2+ is an essential cofactor for virtually all polymerases, restriction enzymes, and many kinases, with the free Mg2+ concentration in PCR reactions typically optimized at 1.5&ndash;3 mM and the magnesium source usually MgCl2 for general use or MgSO4 when sulfate provides a performance advantage for certain DNA polymerases.
Use sodium chloride for ionic strength control in buffers
NaCl at concentrations from approximately 100&ndash;150 mM provides the physiological ionic strength used in most biological buffers, affecting enzyme activity, protein solubility, and protein-protein interaction stringency. Higher NaCl concentrations, such as in high-salt washing buffers, can disrupt weaker electrostatic interactions.
Use calcium chloride as a cofactor for calcium-dependent enzymes
CaCl2 supplies the Ca2+ ions required by calcium-dependent proteins including calmodulin-dependent kinases, C2 domain-containing proteins, and S1 nuclease, and is also used in competent cell preparation where calcium ions facilitate DNA uptake through the bacterial cell membrane.
Use phosphate salts to prepare PBS and other phosphate buffer systems
Monobasic potassium phosphate (KH2PO4) and dibasic sodium phosphate (Na2HPO4) are combined at appropriate ratios to achieve pH 7.4 in PBS, providing the buffering capacity of the phosphate system alongside NaCl and KCl for ionic balance.
Confirm molecular biology grade for PCR and sensitive enzymatic applications
Molecular biology grade inorganic salts confirm the absence of nuclease, protease, and trace heavy metal contamination that could inhibit PCR or other sensitive enzymatic reactions. For non-enzymatic applications like gel electrophoresis buffer preparation, ACS reagent grade is generally adequate.
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<h2>Specifications context</h2>
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Magnesium chloride is particularly critical in PCR optimization because free Mg2+ affects the melting temperature of DNA, the activity and fidelity of thermostable DNA polymerases, and the binding of primers to template, with the optimal MgCl2 concentration varying by template GC content, primer sequence, and reaction conditions. As of 2026, concentrated pre-made MgCl2 solutions at 25 mM or 50 mM supplied sterile with PCR enzyme kits remain standard as a separately added reagent for PCR optimization alongside reaction buffer components.
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Inorganic Salts

Molecular Depot

Key Specifications

Ferrous Sulphate Heptahydrate for Cell Culture

Ferrous Sulphate Heptahydrate for Cell Culture by Molecular Depot is a ferrous sulphate heptahydrate, the hydrated iron(II) salt, of 99.5 per cent purity. It is used as an iron source in culture and fermentation media, as a reagent in iron determination and in oxidative chemistry research. Each unit contains 50 g. MBP supplies Molecular Depot inorganic reagents to research labs in the United States, Canada and worldwide.