Name: EGF Protein, Human Recombinant Animal Free
Brand: Molecular Depot
SKU: B2019324
Price: 1095.00 USD
Availability: InStock

<h2>What are epidermal growth factors?</h2>
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Epidermal growth factor (EGF) and heparin-binding EGF (HB-EGF) are recombinant signaling proteins that bind the EGF receptor (EGFR/ErbB1) to drive epithelial cell proliferation and survival. Related entities include organoid culture media, intestinal stem cell (Lgr5+) maintenance, keratinocyte culture, EGFR signaling pathways, and mammary epithelial cell lines.
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<h2>What you'll find in this category:</h2>
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<ul>
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Recombinant Human EGF: High-purity proteins optimized for stem cell maintenance and primary cell expansion.
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Tissue Regeneration Reagents: Stable growth factors designed for wound-healing assays and tissue culture applications.
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Mitogenic Signaling Tools: Recombinant proteins that support consistent and reproducible cell proliferation studies.
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</ul>
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<h2>How to choose EGF products</h2>
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Recombinant source and expression system
EGF produced in E. coli is the most common and cost-effective option for standard epithelial cell culture supplementation. Mammalian-expressed EGF can matter for glycosylation-sensitive applications, though human EGF is a small, non-glycosylated peptide in its native form, so E. coli-derived recombinant EGF is widely used.
Carrier-free vs. carrier-added formulation
Carrier-free EGF (no BSA) is preferred for animal-component-free (ACF) organoid and stem cell media where bovine protein could affect downstream single-cell or proteomic analysis. Carrier-added EGF offers improved long-term stability for routine epithelial culture supplementation.
Concentration and working range
Most organoid and epithelial culture protocols use EGF at 20&ndash;50 ng/mL in complete media. Confirm the working concentration specified in the published protocol before scaling an order, since under- or over-supplementation can affect proliferation rates and experimental consistency.
HB-EGF vs. standard EGF
HB-EGF binds heparan sulfate proteoglycans in addition to EGFR and is specified in some organoid and wound-healing protocols where matrix-bound signaling gradients are important. Standard recombinant EGF remains the default choice for most general epithelial maintenance media.
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<h2>Specifications context</h2>
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EGF activity is generally reported as ED50 in an EGFR-dependent proliferation or phosphorylation assay, alongside purity by SDS-PAGE (commonly &gt;95&ndash;98%). Pack sizes for academic laboratories typically range from 10 micrograms to 1 milligram, with bulk pricing tiers available for facilities running continuous organoid culture. As of 2026, organoid biobanking protocols increasingly specify carrier-free, low-endotoxin EGF to minimize lot-to-lot variability during long-term passaging.
This category sits alongside Interferons &amp; Interleukins and Fibroblast Growth Factors (FGF) within the broader Growth Factors &amp; Hormones catalog. Laboratories combining EGF with TGF-beta inhibitors for organoid differentiation may also review Transforming Growth Factors.
<a href="https://mbpinc.net/contact-us">Connect with the MBP sales desk</a> today to secure your essential EGF supplies for cell culture and organoid workflows.

Epidermal Growth Factors (EGF)

Molecular Depot

Key Specifications

EGF Protein, Human Recombinant Animal Free

EGF Protein, Human Recombinant Animal Free, catalog B2019324, is an animal-free recombinant human epidermal growth factor (EGF), with typical uses including cell culture media supplementation and EGFR stimulation. Available from MBP, a registered vendor at leading US research institutions.