Name: Dimethyl-Histone H3 (Lys27) Peptide Biotinylated
Brand: Molecular Depot
SKU: B2012656
Price: 695.00 USD
Availability: InStock

<h2>What are peptide-carrier protein conjugates?</h2>
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Peptide-carrier protein conjugates are synthetic peptides chemically coupled to a larger immunogenic carrier protein, most commonly keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA), or ovalbumin (OVA), addressing the fact that a short peptide antigen is often too small on its own to stimulate a strong immune response during immunization. The carrier protein contains many additional epitopes that help stimulate T-cell and B-cell responses, with KLH generally offering the highest immunogenicity of the common options.
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<h2 dir="ltr">What you will find:</h2>
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Carrier-Linked Immunogens: Specialized sequences chemically bound to macromolecular carriers to enhance immunogenicity and stimulate robust antibody production.
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Labeled Tracking Complexes: High-stability peptide chains coupled with functional groups or dyes to enable clear localization and precise visualization within cellular systems.
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Targeted Transport Ligands: Engineered molecular constructs configured to facilitate directional cross-membrane delivery and specific receptor interactions.
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<h2>How to choose a peptide-carrier protein conjugate</h2>
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Choose KLH as the default carrier for highest immunogenicity
KLH is the most frequently used carrier protein for peptide conjugates specifically because of its high immunogenicity relative to other common carrier options, and it does not interfere with subsequent ELISA or western blot experiments since it isn't typically used as a blocking reagent in those assays.
Use OVA as a control carrier to verify antibody specificity
Conjugating the same peptide to ovalbumin instead of KLH or BSA, then testing the resulting antiserum against both conjugates, helps verify that an antibody raised against a KLH or BSA conjugate is actually specific to the peptide itself rather than to the carrier protein.
Confirm your coupling chemistry matches your peptide's added residue
Thiol-maleimide chemistry is the most common, highly specific coupling method, requiring a cysteine residue on the peptide, while glutaraldehyde coupling works through free amino groups and carbodiimide (EDC/WSC) chemistry couples through free carboxyl groups, each suited to a different peptide modification.
Decide on conjugation site based on which terminus carries your target epitope
If the natural epitope sits at one terminus of the peptide, the added conjugation residue, commonly cysteine, should generally be placed at the opposite terminus to avoid disrupting the antigenic region during coupling to the carrier.
Confirm conjugation efficiency and purity before proceeding to immunization
A well-executed peptide-carrier conjugation should achieve high peptide yield with low levels of unconjugated peptide remaining, and confirming this through methods like SDS-PAGE or mass spectrometry analysis helps ensure a quality immunogen before committing to an immunization schedule.
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<h2>Specifications context</h2>
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KLH ranges in molecular weight from approximately 4.5x10^5 to over 1x10^7 daltons depending on its aggregation state, making it substantially larger than BSA or OVA and contributing to its comparatively higher immunogenicity as a carrier protein for peptide conjugation. As of 2026, thiol-maleimide coupling chemistry, using a peptide engineered with an added terminal cysteine, remains the most widely used and reliable method for peptide-to-carrier conjugation across custom antibody production services.
<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the helpful team</a> at MBP today to request a quote.

Peptide Conjugates

Molecular Depot

Key Specifications

Dimethyl-Histone H3 (Lys27) Peptide Biotinylated

Dimethyl-Histone H3 (Lys27) Peptide Biotinylated by Molecular Depot is a histone H3 peptide carrying dimethylated lysine 27 and conjugated to biotin. H3K27 methylation is placed by the PRC2 complex and is associated with transcriptional repression. It is used as a substrate and capture probe in histone methyltransferase and demethylase assays and in binding studies with chromatin reader domains. Each unit contains 100 µg. MBP supplies Molecular Depot protein reagents to research labs in the United States, Canada and worldwide.