With cell culture varieties, dishes, plates, and flasks that exist, knowing the differences between them can be painful but essential. To make matters worse, selecting the incorrect materials can disturb your research. Therefore, we have mentioned the differences between these products to ensure that you pick the one best for your needs. We have also been discussing the fundamental techniques of cell culture to ensure healthy and reproducible cell cultures.
However, MBP INC is the best option if you want top cell culture items for your laboratory. We offer the best cell culture, liquid handling, and sealing film items, including 50ul Conductive Filter Tips – Hamilton, ‘AF-100 aluminium foil sealing film, and many more lab items. You can visit our website and select the best cell culture items for your laboratory.
Cell Culture Dishes
Cell culture dishes are reusable or disposable shallow containers mainly produced to support the propagation and growth of cells in culture. Cell Culture dishes with pre-treated surfaces that allow the attachment of anchor-dependent cells, while untreated cell culture dishes support the suspension cell culture. They are available in multiple sizes in single- or multi-well formats and can be rectangular or round.
Cell culture dishes are mainly made from borosilicate glass or transparent plastics (usually polystyrene or polycarbonate), which allow distortion-free microscopic observation. They frequently come with lids that offer constant gas exchange while protecting the environment.
Cell Culture Plates
Cell Culture plates are usually transparent plates that allow visual assaying and can be either V-shaped, flat, or round at the bottom. They often have lids to protect the samples, which might fit in multiple wells for storage, screening, and experimentation. Cell culture plates are used in laboratories to provide the best conditions for cell culture and the growth of cell cultures.
Cell culture plates are usually coated with an extracellular matrix such as collagen, laminin, fibrin, or modern synthetic hydrogels like polyacrylamide or PEG to allow for better cell attachment, differentiation, and growth.
Cell Culture Flasks
Cell culture flasks are specifically created for the effective growth and propagation of insect, microbial, or mammalian cells. The most common types include flat-sided tissue culture flasks, spinner flasks, and Erlenmeyer flasks. Depending on the application, cell culture flasks are made from borosilicate glass, polycarbonate, or polystyrene and exist in multiple size ranges.Â
Fundamental Cell Culture Techniques – Cell Culture Best Practices
Given below are a few best practices of cell culture. Follow these simple rules to have healthy and reproducible cell cultures.
- Use personal protective equipment (PPE) (laboratory coat/gown, eye protection, and gloves) when handling liquid nitrogen; use full-face visors, thermally insulated gloves, and splash-proof aprons.
- Regularly monitor cultures and media for fungal contamination or gross bacteria signs. This includes a medium that has been bought commercially.
- To cover hair, wear disposable head caps.
- For the tissue culture facility, wear dedicated PPE and keep it separate from PPE worn in the general laboratory atmosphere. Using laboratory coats or different colored gowns makes this simple to apply.
- Handle only a single cell line at a time. Doing this will lowers the cross-contamination possibility by mislabeling etc. It will also decrease the spread of mycoplasma and bacteria by aerosol generation across multiple opened media flasks and bottles and in the cabinet.
- Keep all work surfaces away from clutter.
- Appropriately label reagents like medium, flasks, and ampoules with contents and date of preparation.
- Use a suitable disinfectant (e.g., 70% alcohol) to clean the work surfaces between operations.
- For each cell line in culture, keep separate bottles of media.
- Ensure that incubators, centrifuges, cabinets, and microscopes are cleaned and serviced regularly.
- Before use, examine quality control information for all media and reagents and perfect test performance.
- In all cell culture areas, keep cardboard packaging to the least.
- Test cells for mycoplasma presence regularly.
Conclusion
No matter the nature of your laboratory work, you can be sure that our products are manufactured with a top level of quality that will give you the desired results. At MBPC INC, our cell culture dishes, plates, and flasks come in multiple materials and designs to ensure that our tool collection supplies to everyone. Apart from culture cells, we also offer 200ul Low Retention Filter Tips, PCR consumables, reagents, and many more. For more info, take a look at our online store.
