Name: Biotin Conjugated to 5-fluorescein
Brand: Molecular Depot
SKU: B2012427
Price: 495.00 USD
Availability: InStock

<h2 data-section-id="pu6krb" data-start="0" data-end="35">What are fluorescent conjugates?</h2>
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Fluorescent conjugates are pre-made fluorophore&ndash;antibody or fluorophore&ndash;protein products that are supplied ready to use, without requiring any separate labeling reaction. These include directly conjugated primary antibodies, fluorescent secondary antibodies, and fluorescent streptavidin or other affinity protein conjugates. They are designed for immediate use in assays such as flow cytometry, immunofluorescence, immunohistochemistry, and ELISA. Related entities include direct vs. indirect immunofluorescence detection, tandem dye conjugates, multiplex flow cytometry panel design, and lot-to-lot consistency in antibody labeling.
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<h2 dir="ltr">What you will find: </h2>
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Fluorophore-Linked Macromolecules: High-purity primary molecules and biological complexes chemically cross-linked to intense reporters to enable direct visual localization, rapid tracking, and reliable structural identification across various assay formats.
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Secondary Immunological Probes: Calibrated target conjugates prepared to bind primary markers with high specificity, delivering robust signal amplification, reduced cross-reactivity, and clear population differentiation in multi-tier screening setups.
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Calibrated Detection Complexes: Specialized molecular linkages engineered to provide stable light emission, optimal quantum yields, and highly reproducible baseline metrics during demanding quantitative analysis and high-throughput cellular screening.
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<h2 data-section-id="1295bux" data-start="824" data-end="871">How to choose fluorescent conjugate products</h2>
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Direct vs. indirect detection strategy
Directly conjugated primary antibodies bind the target antigen and are immediately detectable, making workflows simpler, faster, and typically cleaner with lower background. Indirect detection uses an unconjugated primary antibody followed by a fluorescent secondary antibody, which increases signal amplification because multiple secondary antibodies can bind to each primary antibody, improving sensitivity for low-abundance targets.
Tandem dye conjugates for panel expansion
Tandem dye conjugates combine a donor and acceptor fluorophore to produce emission at a wavelength distinct from either dye alone. This expands the number of usable channels in high-parameter flow cytometry panels. However, tandem dyes can be more sensitive to photobleaching and degradation, so proper storage and handling are important for consistent performance.
Species cross-reactivity for secondary antibodies
Fluorescent secondary antibodies are generated against specific host species such as mouse, rabbit, goat, or rat. They may also show defined cross-reactivity profiles with related species. Correct matching between the secondary antibody and the host species of the primary antibody is essential to avoid non-specific binding and background signal.
Lot-to-lot consistency for quantitative panels
For multiplex or quantitative assays, consistency in fluorophore-to-protein ratio and binding activity between production lots is important. Variations between lots can affect signal intensity and comparability across experiments, so matched lots or validated lot consistency data are often preferred for long-term studies.
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<h2 data-section-id="1fljvas" data-start="3394" data-end="3419">Specifications context</h2>
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Fluorescent conjugates are defined by excitation and emission maxima, fluorophore-to-protein (F:P) ratio, and validated application performance across techniques such as flow cytometry, immunofluorescence, and ELISA. Quality control typically includes lot-specific validation of binding activity, fluorescence intensity, and stability. Many conjugates are stored at 2&ndash;8&deg;C with protection from light and are sensitive to repeated freeze&ndash;thaw cycles.&nbsp;
This sub-category is part of MBP's fluorescent reagents catalog alongside fluorescent labels and dyes, which are used for custom in-house labeling when pre-made conjugates are not available. MBP&rsquo;s specialist team can help confirm conjugate availability, species compatibility, and spectral fit for a specific multiplex panel before order placement.
<a href="https://mbpinc.net/contact-us">Contact the helpful team</a> at MBP today to request a quote.

Fluorescent Conjugates

Molecular Depot

Key Specifications

Biotin Conjugated to 5-fluorescein

Biotin Conjugated to 5-fluorescein by Molecular Depot is a conjugate of biotin and 5-fluorescein. Joining biotin to a fluorophore gives a probe that is both fluorescent and capturable by streptavidin. It is used to titrate and block avidin and streptavidin binding sites, as a fluorescent tracer in binding assays and as a control in biotin-streptavidin systems. Each unit contains 1 mg. MBP supplies Molecular Depot protein reagents to research labs in the United States, Canada and worldwide.