Name: BioCoupler®
Brand: Plant Cell Technologies
SKU: PCT-BIOCOUPLER
Availability: InStock

<h2 dir="ltr"> What are NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; Systems are compact, single-use, multi-layer cell culture vessels manufactured from USP Class VI polystyrene for large-scale adherent cell culture. The BioFactory provides the growth kinetics and cell attachment characteristics of standard T-flasks, scaled up to growth areas from approximately 636 cm&sup2; (1-layer) to 25,440 cm&sup2; (40-layer) in a single vessel &mdash; making it the scale-up path from T-flask and roller bottle culture for vaccine production, monoclonal antibody manufacturing, viral vector production (lentivirus, AAV, adenovirus), and biopharmaceutical protein expression from adherent cell lines.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>BioFactory systems: Multi-layer sterile cell culture vessels available with different chamber configurations, plug seal cap options, transfer caps, vent filters, and frost-resistant models for large-scale adherent cell culture.</li>
<li><a href="https://mbpinc.net/consumables/cell-culture/biofactory-systems/biofactory-system-accessories">BioFactory System Accessories:</a> Adaptor caps, overcaps, holders, vent filters, accessory packages, tubing sets, and preconfigured kits designed to support media handling and system operation.</li>
<li>Sterile fluid transfer components: CPC connectors, T- and Y-shaped connectors, sealing plugs, adaptor connectors, hose clamps, funnels, and filter/tubing assemblies for closed liquid transfer workflows.</li>
<li>Bioprocess tubing: Sterile C-Flex&reg; welding hose, SPT-50 hose, and inlet/outlet tubing compatible with BioFactory systems and media transfer applications.</li>
<li>Preassembled BioFactory configurations: Ready-to-use systems equipped with integrated vent filters, transfer tubing, or specialized cap assemblies to streamline cell culture setup.</li>
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<h2 dir="ltr"> How to Choose the Right BioFactory&trade; Layer Count</h2>
 Matching BioFactory Scale to Production Stage The 1-layer BioFactory (&asymp;636 cm&sup2;) is equivalent in surface area to approximately 8&ndash;9 standard T75 flasks &mdash; used for proof-of-concept scale-up studies transitioning from T-flask monolayer culture. The 2-layer BioFactory (&asymp;1,272 cm&sup2;) is the standard for pilot-scale viral antigen production and early-phase process development. The 5-layer (&asymp;3,180 cm&sup2;) and 10-layer (&asymp;6,360 cm&sup2;) formats suit mid-scale production runs for research-grade vaccine lots, transfection-derived protein, and pre-clinical viral vector batches. The 40-layer (&asymp;25,440 cm&sup2;) format is an industrial-scale format for commercial vaccine, mAb, and biopharmaceutical production. Closed Liquid Transfer and Contamination Reduction NEST BioFactory&trade; systems feature a narrow-mouth cap port compatible with aseptic tubing for closed liquid transfer &mdash; media, wash buffers, trypsin, and harvest supernatant can be introduced and removed through the tube port without opening the vessel. This closed-system operation eliminates the open-pour steps that create contamination risk in multi-layer roller bottle workflows and enables BSL-2 containment of pathogen-bearing cultures without biosafety cabinet&ndash;dependent open-handling. Wide-mouth caps allow direct media pouring for non-closed workflows. TC Treatment and Cell Compatibility All NEST BioFactory&trade; layers are TC-treated by vacuum plasma using the same surface treatment process as NEST cell culture T-flasks. NEST has published VERO cell culture data showing that cell morphology, adherence, and growth kinetics in the BioFactory are equivalent to those in standard T-flasks &mdash; cell attachment density, proliferation rate, and morphology are not altered by the scale-up. This linear scalability is the primary engineering advantage of multi-layer vessels over microcarrier bioreactors and packed-bed systems for adherent cell&ndash;derived products requiring 2D culture validation. Ultrasonic Welding and Particle Generation The BioFactory layers are joined by ultrasonic welding without chemical adhesives or extrinsic ingredients. NEST's welding line design minimizes particle generation at the weld joints, an important quality attribute for pharmaceutical production contexts where subvisible particle counts in harvest media must meet compendial limits. Automation and GMP Compatibility NEST BioFactory&trade; systems are compatible with automated media handling equipment. Multiple BioFactory units can be connected in series through the tubing ports for further scale-out. The double-bagged sterile packaging format supports GMP cleanroom entry requirements. NEST provides validation documentation packages (sterilization data, extractable/leachable certificates, biocompatibility reports) supporting GMP authorization for industrial cell culture production. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; Systems are manufactured from USP Class VI polystyrene in a Class 10,000 (ISO 7) dedicated cleanroom. All culture surfaces are TC-treated by vacuum plasma. Temperature range: approximately &minus;86 &deg;C to +64 &deg;C. E-beam sterilized to SAL 10⁻⁶; non-pyrogenic; double-bagged sterile packaging. Vent caps contain 0.22 &micro;m hydrophobic filters for gas exchange without contamination. VERO cell culture validation data available from NEST Scientific: seeding at 5 &times; 10⁴/mL in 2-layer BioFactory (400 mL) shows robust, even adherence and growth kinetics matching T-flask controls after 3 days. Cell lines validated: Vero, MRC-5, 2BS, 293T, L-929, and equivalent adherent production lines. NEST Scientific BioFactory&trade; systems across all layer configurations are carried by MBP. 
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Biofactory Systems

Plant Cell Technologies

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BioCoupler®

BioCoupler® (BC1–BC48) by Plant Cell Technology is an autoclavable temporary immersion bioreactor coupler that joins two 70 mm regular-mouth jars for liquid-medium plant propagation without electricity. Variant catalog numbers: BC1 (1 BioCoupler), BC6 (6 BioCouplers), BC12 (12 BioCouplers), BC24 (24 BioCouplers), and BC48 (48 BioCouplers). Available from MBP, a registered vendor at leading US research institutions.