Lab, Medical, Tubes

An Introduction to Cell Cultures

Cell culture is a technique where cells from an organism are removed and made, then cultivated in an artificial environment that is favorable to their development. Their removal can be either direct through enzymes or mechanical means or they. Or it can be derivative by taking cells from an already existing cell line or cell strain. There are three main types of cell cultures that exist:

 

Primary Culture

Primary culture is the initial process of isolating cells from the organism and then preparing them for further processes by proliferating them throughout a substrate. Once that is achieved, the cells are then subcultured, meaning they are transferred to another container in which the environment is artificially tailored to speed up and enhance the development of the culture.

 

Secondary Culture

In the process of a secondary culture, cell lines have been influenced by human telomerase transcriptase (also known as hTERT), which is an enzyme that modifies and alters the phenotypic properties of cell culture, allowing it to divide indefinitely. Once achieved, the culture will keep expanding, allowing researchers to get more and more samples. This process is also known as cell immortalization and can only be achieved with certain types of cells and in only certain circumstances.

 

Continuous and Finite Cell Lines

Cells that do not undergo the immortalization process don’t proliferate and expand indefinitely and, as such, only remain as finite cell lines. But those that do go through the transformation, as mentioned under the previous heading, expand indefinitely. The latter is then known to be continuous cell lines.

 

Conditions for Cell Culture

Ultimately, the conditions needed to culture a cell depend on the type of cell it is and where it was derived from. But there are certain conditions that are universal in cell culture:

 

  1. Substrate: It is a medium in which the cells are suspended. These substrates are a medium that contains all the essential nutrients such as carbohydrates, amino acids, vitamins, minerals and such that a cell culture needs to expand.
  2. Growth Factors: These are proteins that will stimulate the growth of the cells. These proteins help in cellular division and differentiation.
  3. Hormones: Hormones also help the cell culture expand but more importantly, they control and regulate the growth of the cell culture.
  4. Gases (O2, CO2): These gases are essential to maintain the health and pH level of the culture due to the reactions that they have with the medium and the cells themselves.
  5. A Regulated Environment: All external factors such as room temperature, humidity levels, and other factors are also important.

Though it depends on the type of cell, cells require one of two kinds of substrates to develop, some cells require a solid or semi-solid substrate environment while others can be suspended in a liquid substrate environment. 

 

Cryopreservation

If, after the process of subculturing, there remains a surplus of cells that are about to go unused, then discarding them is not recommended. Instead, it is recommended to cryopreserve them (store them in -130 Degree Celsius temperatures) in a protective agent to keep them safe until they are required for further use.

 

MBP Inc offers different products for their storage and other storage, such as cryogenic vials, sealing film for cold storage, Kingfisher Plastics deep-wells, and more.

 

Applications

Cell culture is an essential and major tool in the field of microbiology and cellular biology. They allow researchers and doctors to study the physiology and biochemistry of cells. In research, these cells can be studied for how they are built and how various environmental and internal factors affect them, their lifespan and health. They can also be tested for how they are affected if drugs or other toxic compounds are imposed upon them. In the medical field, cultures are important to detect diseases, develop vaccines and other medical compounds. Cell cultures have incredibly versatile uses, and they themselves are quite versatile due to their endurance and ability to reproduce.

 

Conclusion

The complicated process of cell cultures is made easy by modern technology and equipment. MBP Inc is a company that aims to make processes like this more straightforward and more convenient for researchers and doctors. We sell equipment for the cell culture process, such as cell culture dishes, flasks, plates, and Erlenmeyer flasks. We also sell a whole host of other products such as pipettes and filter tips, 200ul Low Retention Filter Tips being a good example. So, if you are looking for someone to supply your lab with essential products and equipment, then MBP Inc is the place for you. Order today.

 

Bibliography

[1]  “Introduction to Cell Culture,” [Online]. Available: https://www.thermofisher.com/pk/en/home/references/gibco-cell-culture-basics/introduction-to-cell-culture.html. [Accessed 6 12 2021].

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