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When it comes to laboratory work in the field of microbiology, pipettes and pipettors are quite essential. They can make the transfer of liquids convenient and precise. Let’s take a look at the things you need to know about serological pipettes.
Types of Serological Pipettes and Their Uses
First and foremost, serological pipettes are used to transfer liquids [1]. You can measure the amount of liquid in one pipette through the measurement scale imbued into the side of a pipette. It measures liquids in milliliters (ml). Pipettes are especially useful because of the accuracy with which you can transfer a liquid.The following processes use serological pipettes.
- Mixing Suspensions
- Adding reagents to chemical solutions to being a reaction
- To transfer cells or DNA/RNA structure for analysis.
Serological pipettes are also known as blow-out pipettes. That is due to their design and the fact that to get the full volume of any liquid, you would have to blow it out of the tube. As such, rubber bulbs are attached to the head of the pipette to ‘blow-out’ the excess.
Pipette tips are generally signified by double rings that encircle the tube. The use of these pipettes is easy as you only need to squeeze the rubber bulb to get all the liquid out of the pipette.
Kinds of serological pipettes
These kinds of pipettes are usually only designed using either glass or polystyrene plastic. And it is recommended to only use a pipette once before disposing of it due to hygiene reasons. There are three different kinds of pipettes.
Open-end Pipettes
These pipettes are great for measuring liquids with high viscosity due to their exposed end. Due to how quickly they can fill and empty, they are perfect to be used for thick fluids like oil, paint, sludge and others.In the way of protection, they come with fibre filter plugs that reduce the chances of fluid contamination. They also have non-pyrogenic and sterilized properties. They also come in specially designed protective packaging to further prevent any chances of contamination.
We offer 10ml serological pipettes as well as 25ml and 50ml variations.
Bacteriological Pipettes
When the testing of fluids with bacteria is involved, such as milk and other dairy products, then bacteriological pipettes are considered the best option. They are also single-use, non-pyrogenic pipettes that are sterilized using gamma radiation.
Aspirating Pipettes
Aspirating pipettes, as the name suggests, are designed to be open and ready to mix any fluids through a vacuum or a pipette aspiration process. Also, single-use for hygiene reasons, non-pyrogenic, sterilized through gamma rays and come in protective packaging.
Reading the Volumes of Serological Pipettes
Reading the measurements of these serological pipettes is best done by someone who has expertise in this area. There are some essential factors to take into consideration when reading these measurements:
- Once you have the pipette fully filled, only then should you take the meniscus reading.
- It is recommended to hold the pipette at eye level to obtain any reading.
- Hold your pipette at an exact right angle to the floor.
- Make sure to take multiple measurements to eliminate the margin of error.
Dispensing of Fluids through a Serological Pipette
If you’re thinking about transferring fluids through the mouth pipetting method, then it is strictly not recommended due to the chance of accidentally consuming those fluids. It is considered a primitive and outdated method that can be harmful to the tester. It is best to use the rubber bulb attached to the head of the serological pipette. But it is best to remember that dispensing the liquid through the rubber bulb isn’t fully accurate.
Benefits of Using a Serological Pipette
- These kinds of pipettes are optimized optically. This provides high accuracy of volume and measurements.
- Due to their design, they are highly sterilized and easily disposable, meaning you don’t have to worry about contamination or disposal.
Conclusion
Serological pipette tips have many uses in the field of microbiology and allow testers accurate fluid dispensation with the least chances of any contamination. These aspects make these pipettes highly valued and useful in their environments.MBP Inc offers the best quality microbiological products in the current market. You can find equipment such as freezers and centrifuges, and other products like Filtered and Non-filtered pipette tips, conductive filter tips for Tecan, and a host of other equipment that can be useful to you.
Bibliography
| [1] | "Types of Serological Pipettes and Their Uses," 17 January 2020. [Online]. Available: https://www.genfollower.com/serological-pipettes-types-uses/. [Accessed 6 December 2021]. |
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