3D cell models have become famous for learning complex biological impacts. They represent the tissue microenvironments, cell-to-cell relations, and natural methods that happen in vivo affecting tissue functionality and diseases. An organoid displays realistic micro-anatomy, a miniaturized and essential form of an organ formed in vitro in three dimensions.
Their skill to self-manage and intimate functional organ cell categories is thought to be better represented within living biology than in 2D monolayer cell cultures. For broader use to explore drug progress, barriers are being broken down with advanced technologies because these models’ complications can be obstacles. Mechanical procedures of cell culture and imaging to monitor the progress illustrate the complex responses in 3D organoids due to advances in cell technologies.
- Automated cell culture organoids High-definition imaging procedure
- Drug detection and expansion of Organoid applications
- Pulmonary (lung) organoids
- Intestinal (gut) organoids
- Brain (cerebral) organoids
- Patient-Derived Organoid (PDO)/Tumoroids
- 3D biology workflows Automation protocol
Automated cell culture organoids High-definition imaging procedure
For the downstream study, organoids for disease modelling and valuation of compound effects and the quality of images are essential. High-performance, automated imaging and analysis solutions are necessary for fully quantitative and robust assessment of organoid phenotypic changes and growing the throughput of experiments and screens.
Confocal microscopy is a method that uses lasers and fluorescence to make a three-dimensional image of a sample. It enables practical imaging of 3D objects for spheroids, organoids, and organ-on-a-chip models.
The imagexpress micro confocal is a High-Content Imaging System with eight imaging channels containing a seven-channel laser light source. It enables highly multiplexed assays and penetrating spinning disc confocal technology deeper into thick tissue samples for sharp images. Its water immersion objective signal to increase the noise ratio, improve z-resolution, and reduce optical aberrations for more brilliant, crispier images
Traditional image analysis methods are complicated and time-consuming due to their manual process. There is a possibility of human mistakes and bias occurring. Therefore, the invitation to advanced image analysis tools and machine learning are developed. Advanced image analysis software provides information about changes in phenotypes.
Organoid applications for drug discovery and development
In cancer research, organoids are essential in neurobiology, stem cell research, and drug discovery. They allow for the enhanced modelling of human tissues. These 3D microtissues deliver scientists’ details into the human growth and disease system.
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Lung organoid cultures:
A 3D tissue-engineered mini lung recapitulates the airway’s structural and functional elements, such as ciliary beating, regeneration, alveolar structure, and mucus emission. They occupy potential for a wide range of applications such as drug screening and disease modelling.
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Intestinal (gut) organoids
They are the first 3D organoid models to recap structures in the intestinal lumen and on the surrounding intestinal epithelium. Scientists study intestinal cell biology, revival, discrepancy, and disease phenotypes, including effects of specific mutations, microbiome, or inflammation processes from the cell composition and arrangement of the epithelium, making intestinal organoids.
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Brain (cerebral) organoids
Brain organoids are 3D tissue models that signify multiple sections of the brain. They resemble structures of various brain regions. Cerebral 3D organoid technology is rapidly growing and has great potential for learning about human brain progress and neuronal diseases. They are used for testing the impacts of compounds and genetic mutations. This approach assists in studying pharmaceutical drugs, functional genomic applications, and the effects of toxins.
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Patient-Derived Organoid (PDO) /Tumoroids
They are 3D cultures that are produced from primary tumours of specific patients. Tumoroids are a precious method tool for drug expansion, cancer research, and personalized medicine.
3D biology workflows Automation protocol
Due to the complication of organoids, more cultured 3D imaging and analysis techniques are required to describe the unsolved biological secrecies precisely and efficiently. Therefore, automated confocal imaging systems and 3D image analysis software are usually used to assist investigators in modernizing their workflow and obtaining efficient results.
Conclusion
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Bibliography
Sirenko, O. (2022, June 15). moleculardevices. Retrieved from Molecular Devices: https://www.moleculardevices.com/lab-notes/cellular-imaging-systems/how-automated-organoid-cell-cultures-are-developed-imaged-and-analyzed
