It is a real battle to load the deep well plate correctly. You can easily slip off information on how many pipettes or plates you have loaded before in your lab.
It is very simple to add volumes to the incorrect well or row. You waste hours loading the erroneous sample into many wells, or perhaps you are confident that you have done the correct thing.
Starting over is always annoying and time-wasting because your time is priceless. Moreover, nobody can afford to waste precious reagents. A lot of time is wasted by having to re-make reagents and re-mix. Above everything else, your information is invaluable.
How To Load Deep Well Storage Plates Without Error
An often-overlooked yet crucial component of laboratories and research facilities everywhere, Deepwell Storage Plates can be used for both short- and long-term storage, as well as the preparation and mixing of samples. You can have a round or square base.
Use the following suggestions from the work of others to improve your laboratory practices. Listed here are the most effective methods and tools to load deep-well plates without error:
Mindset And Focus
Errors are more likely to occur under adverse conditions, such as fatigue, stress, or distraction, as is the case with any task. Don’t stress out about finishing quickly. Put the brakes on and give each step a little more consideration. Also, keep your mind clear. Some lab scientists will even post a “No talking” sign if they are in the middle of a vital task experiment. However, soothing music is encouraged if you require some background noise while you work.
Match Pipette Tips To Wells
For deep well plates, it’s ideal to use a brand-new pipette box. Concurrently pair the well with the filter pipette tips. If you want to avoid disrupting your system if you run out of tips, you should keep a spare box on hand. One can keep track of the number of wells using pipette tips.
You must make a master mix Excel document and deep well plate diagrams. Primers and samples are identified by name in individual wells. It will help if you use color coding to organize your master mixes and ensure that each primer set is labeled correctly. In the laboratory, take this sheet with you and mark it off as you go. A post-it note with the necessary reagent amounts written on it could serve as a sample key to refer to when you load the sample.
Tape Is Necessary
Wrap up the entire plate in tape except for the spot where the content is now being added. Proceed over the plate, repositioning the tape after each completed section. You can remain on track by labeling your tape (for example, A–H, 1–12).
Hold Firm the Process
Don’t abandon a failing strategy in the middle of its implementation. You can make alterations before or after the task, but not in the middle of it. It is wiser to complete one strategy; in case it fails, you should go for its reconciliation once you get the complete picture of the mishap.
Practice Your Method
If you choose a particular method, stick to it. Learning these procedures by heart will take time, but in the long run, you should see considerable improvements in your work and much less frustration on the job. Make a list of steps you carry out and keep marking them to double-check the procedure.
There is a variety of methods of dealing with deep-well storage. Where they are handy, they can be a big disaster if followed with the wrong procedures.
So we have discussed some of the useful methods above to help you out saving the expensive reagents and mixes from going to waste.
To order deep well storage plates and filter tips for your laboratory, contact MBP Inc.. We also have a comprehensive selection of pipette tips, including standard, non-filter, conductive, eco-friendly tips, and a wide variety of different volumes, including but not limited to 20ul Filter Tips, 50 ul, 100ul, 200ul & 1000ul Filter Tips.