Name: 5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB
Brand: Molecular Depot
SKU: B2010677
Price: 250.00 USD
Availability: InStock

<h2>What are biochemical reagents?</h2>
&nbsp;
Biochemical reagents are biologically relevant chemical compounds used in laboratory research: vitamins and cofactors that support enzymatic reactions and cell growth, buffer solutions that maintain pH and ionic strength for protein stability and enzymatic activity, amino acids used as cell culture supplements or labeled standards, and other biologically active small molecules used across molecular biology, cell biology, and biochemistry applications. Unlike application-specific reagents organized by workflow, biochemical reagents are general-purpose compounds that appear as components or supplements across many different experimental contexts.
&nbsp;
<h2 dir="ltr">What you will encounter:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/buffers-and-solutions" title="Buffers &amp; Solutions:">Buffers &amp; Solutions:</a> Standardized formulations that stabilize pH, comprising essential TRIS, DTT, and particular TE storage systems.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/lipids-and-lipid-derivatives" title="Lipids and Lipid Derivatives:">Lipids and Lipid Derivatives:</a> Effective fatty substances and structural lipid components refined for membrane studies and transfection techniques.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/polymers-and-peg-derivatives" title="Polymers &amp; PEG Derivatives:">Polymers &amp; PEG Derivatives:</a> Premium functional polymers designed for precise chemical conjugation, drug delivery assessment, and protein stabilization.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/amino-acids-and-derivatives" title="Amino Acids &amp; Derivatives:">Amino Acids &amp; Derivatives:</a> Refined peptide components and modified amino derivatives for tailored culture media compositions.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/enzyme-inhibitors" title="Inhibitors of enzymes:">Inhibitors of enzymes:</a> Extremely specific blocking agents such as PMSF intended to stop proteolysis and safeguard important biological target proteins.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/enzymes" title="Enzymes:">Enzymes:</a> Catalytic proteins with structural stability and digestive enzymes tailored for effective substrate cleavage and cellular breakdown.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/vitamins">Vitamins:</a> Crucial organic micronutrients and coenzymes designed to promote microbial proliferation and the development of specialized tissue cultures.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/nanoparticles-and-advanced-nanomaterials" title="Nanoparticles &amp; Advanced Nanomaterials:">Nanoparticles &amp; Advanced Nanomaterials:</a> Precisely designed microstructures created for enhanced molecular tracking, imaging enhancement, and targeted delivery of substances.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents/carbohydrates-and-derivatives" title="Carbohydrates and Derivatives:">Carbohydrates and Derivatives:</a> Refined sugar compounds and metabolic inducers such as IPTG and L-Arabinose for regulated gene expression systems.
</li>
</ul>
&nbsp;
<h2>How to navigate Biochemical Reagents</h2>
&nbsp;
Start with Vitamins for fat- and water-soluble vitamins and cofactors
The Vitamins subcategory covers individual vitamin compounds &mdash; fat-soluble (A, D, E, K) and water-soluble (B1&ndash;B12, C) &mdash; used as cell culture media supplements, biochemical standards, or research tools for studying specific metabolic pathways.
Check Buffers &amp; Solutions for general-purpose biochemical buffers
Buffers &amp; Solutions covers the commonly used biochemical buffer systems &mdash; HEPES, PBS, Tris, PIPES, MOPS, and others &mdash; for applications like protein storage, cell lysis, enzyme reactions, and chromatography, distinct from the gel electrophoresis-specific running buffers in the Electrophoresis category.
Confirm purity grade is appropriate for your biochemical application
Cell culture supplementation requires vitamins and amino acids at cell culture or molecular biology grade to minimize trace contaminants that could affect cell viability or growth, while enzymatic and biochemical assay applications require defined, characterized purity and lot-to-lot consistency.
Use biochemical standards for quantitative assay calibration
Research-grade vitamins and amino acids with documented purity can serve as calibration standards for quantitative analytical assays, where the purity grade and lot certificate of analysis are required to calculate actual concentrations reliably.
Consider ready-to-use solution formats for frequently used buffers
Pre-made, sterile buffer solutions at working concentration eliminate preparation and pH adjustment steps for frequently used biochemical buffers, improving reproducibility and saving preparation time compared with making each buffer from powder per use.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Biochemical reagents for cell culture use, including vitamins and amino acids, are typically produced to cell culture grade or equivalent, confirming absence of endotoxins and other trace contaminants at levels that could affect cell viability. As of 2026, pre-made, sterile-filtered buffer solutions at defined concentrations continue to be widely adopted across research labs that value preparation-free convenience and lot-to-lot consistency for commonly used buffers like PBS and HEPES.
<a href="https://mbpinc.net/contact-us">Reach out to the supportive team at MBP</a> today to obtain a quote for your specific research needs.

Biochemical Reagents

Molecular Depot

Key Specifications

5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB

5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB by Molecular Depot is a highly pure, water-soluble reagent for derivatizing sulfhydryl groups (Ellman's reagent, DTNB). DTNB reacts with free thiols to release a yellow product. It is used to measure free sulfhydryl groups in proteins and peptides and in cholinesterase activity assays. Each unit contains 1 g. MBP supplies Molecular Depot research reagents to research labs in the United States, Canada and worldwide.