

5-bromo-6-chloro-3-indoxyl-2-acetamido-2-deoxy-β-D-glucopyranoside by Molecular Depot is a chromogenic substrate for N-acetyl-β-D-glucosaminidase that yields a magenta precipitate on cleavage. The released indoxyl oxidises and dimerises in air to an insoluble indigo dye, so the colour forms as a precipitate where the enzyme is rather than diffusing into the medium. It is used to detect N-acetyl-β-D-glucosaminidase on agar plates and in gels, in microbial identification media and enzyme localisation and in histochemical staining. Each unit contains 50 mg. MBP supplies Molecular Depot carbohydrate reagents to research labs in the United States, Canada and worldwide.