

5-bromo-4-chloro-3-indoxyl-β-D-cellobioside by Molecular Depot is a chromogenic substrate for β-D-cellobiosidase that yields a blue precipitate on cleavage. The released indoxyl oxidises and dimerises in air to an insoluble indigo dye, so the colour forms as a precipitate where the enzyme is rather than diffusing into the medium. It is used to detect β-D-cellobiosidase on agar plates and in gels, in cellulase screening and microbial identification media and in histochemical staining. Each unit contains 50 mg. MBP supplies Molecular Depot research reagents to research labs in the United States, Canada and worldwide.