

4-nitrophenyl-β-D-maltopyranoside (p-nitrophenyl-β-D-maltoside) by Molecular Depot is a 4-nitrophenyl-β-D-maltopyranoside, also written p-nitrophenyl-β-D-maltoside, a chromogenic substrate for α-amylase and maltase that gives a yellow solution on cleavage. The nitrophenol released on cleavage absorbs strongly at about 405 nm in alkali, so the reaction is read directly in a spectrophotometer or a plate reader. It is used as the substrate in colorimetric α-amylase and maltase assays, in amylase determination and carbohydrase screening and in enzyme assay development. Each unit contains 25 g. MBP supplies Molecular Depot carbohydrate reagents to research labs in the United States, Canada and worldwide.