Have a look around any lab, and you will find a variety of pipettes being utilized in various applications. One type of pipette that serves a great deal of purposes in labs is a serological pipette. They help measure and transfer liquids in millilitres ( 1-50ml).
Serological pipettes come in three varieties, each of which has a distinct function and is utilized while conducting varying tests and experiments. Read ahead in this article to learn more about these three distinct serological pipette.
Use of Serological Pipettes in Laboratory
The following are the main applications for serological pipettes:
- Merging a suspension.
- Mixing chemical liquids and reagents.
- Moving cells for expansion or econometric findings.
- Reagent layering produces gradients of higher densities.
3 Types of Serological Pipettes
Open-end pipettes are mainly created with an open end meant to be used with fluids having lower permeability. LTS pipette tips can be used on the open end for samples that are required to be covered.
The pipettes’ open-end design makes it quick and simple to remove excess samples and distribute thick liquids. They are suitable for use with oils, cosmetics, paints, sludge, food applications, and tissue culture applications due to their quick fill and release rates. These polyethylene plastic open-ended pipettes offer a more secure option than glass pipettes.
The fibre filter plug at the pipette’s tip greatly reduces the aqueous and liquid vapour contaminants of the pipettors and samples. Gamma radiation is used to sterilize these non-pyrogenic pipettes, which are packaged individually in thermoplastic paper/plastic peeling packing.
The diameters of these serological pipettes are 1 mL, 2 mL, 5 mL, and 10 mL. These pipettes are a great option for effective liquid handling because they are colour-coded, complying with industry-standard ASTM E1380 with their simple labelling.
Bacteriological pipettes, also known as milk pipettes or bacteriological pipettes, are generally used to examine dairy products. They adhere to the American Public Health Association (A.P.H.A.) criteria for the inspection of milk products. There are two sizes of these polystyrene bacteriological pipettes: 1.1 mL and 2.2 mL.
These single-use, gamma-irradiated, non-pyrogenic bacteriological pipettes are individually packaged in moulded paper/plastic peel packing. They use a fibre filter to shield samples from fluid and liquid vapour contamination, as well as to protect the internal workings of the pipette. Bacteriological pipettes comply with ASTM E934 requirements and are certified to deliver (TD) at +/- 2%.
In contrast to bacteriological and open-ended pipettes, aspirating pipettes are completely clear and free of gradations. They are utilized in a variety of applications, such as vacuum or pipette aspiration processes, and are used to transport and mix several liquids. In the lab, these solitary, non-plugged, non-pyrogenic polystyrene pipettes are a far safer option than glass Pasteur pipettes.
To avoid contamination, these aspirating pipettes are packaged individually in thermoplastic paper or plastic peeling. These gamma-irradiated pipettes, which have been sterilized, have a sterile assurance level of 10–6.
How to Use Serological Pipettes to Read Measurements?
Lab technicians and staff members responsible to carry out research and experimental applications may need serological pipettes and LTS pipette tips frequently. For accurate results, one needs to have a basic understanding of how to read volumes and measure fluids in them. Here are some things to remember when using a serological pipette:
- Once the pipette is filled, the bottom portion of the droplet should be observed. At the apex of the liquid column, the vortex develops.
- To get an accurate reading, hold the pipette close to your eyes.
- Holding the pipette at the most ideal angle to the ground is required.
What Advantages Do Serological Pipettes Offer?
Whether you’re using an open-ended serological pipette, aspirating or biological pipette, you will be able to measure and transfer different types of samples easily. Here are some advantages serological pipettes offer.
- They have been optically improved.
- They deliver increased volume accuracy.
- They are pre-sterilized and disposable. They come in packs of 25 to 50 and are made to accommodate pipet-aids and motorized pipettes.
You should now have a clear perspective of the distinctive qualities of each type of pipette after reading about the three possible serological pipette types. Various sizes of pipettes can be utilized using the same pipette processor for a range of experimental tests, adding versatility and simplicity.
Get in touch with Molecular Biology Products if you want to stock up on serological or aspirating pipettes. Practice various liquid sample applications and storage with the most market-competitive molecular biology equipment.