DNA extraction is performed to obtain isolated molecules with a high degree of purity and to be able to use them in scientific research, medicine or forensic science.
You can find diverse DNA extraction methods, including Proteinase K, incubation of lysate for use, sodium iodide DNA extraction method, and so on. With the diversity of currently known methods, DNA can be extracted from many substrates such as blood, saliva, urine and other body fluids, living tissues, cell cultures, and amniotic fluid, among others. This blog highlights some methods for DNA extraction. Keep reading!
1. Use of Proteinase K and sodium dodecyl sulfate
The procedure known as Proteinase K – sodium dodecyl sulfate (PK-SDS) is one of the most used for DNA extraction. The reason is that the digestion of the sample can be performed with Proteinase K that is activated by dodecyl sulfate of sodium (Qamar et al., 2017).
Proteinase K is a solution that inactivates DNAses present in the cell lysate using SDS anionic detergent. Proteinase K is also used in other methods for the digestion of protein and other contaminants in cell lysate. (Qamar et al., 2017)
2. Sodium iodide DNA extraction method as a chaotropic agent in a single tube
You can find several DNA kits available to researchers and companies that use sodium iodide as a chaotropic agent, which have the advantage that the extraction is carried out in a single micro centrifuge tube. Sodium iodide, in addition to causing cell lysis, breaks the hydration layer that surrounds the DNA and makes the negative charges of the phosphate groups of the DNA more exposed. (Hamilton, 2001)
In these kits, after treating the sample with sodium iodide in the same tube, anionic detergents are added, such as sodium N-lauroyl sarcosinate, Proteinase K and/or other reagents that allow separating the proteins and lipids contained in the DNA biological samples (Hamilton, 2001).
3. Anion exchange for DNA extraction from cell lysate
Anion exchange solid phase chromatography is based on the interaction established between the negatively charged phosphate groups of DNA and the molecular matrix with which the column is compacted. This interaction causes the DNA molecules to be retained in the stationary phase of the column and can be separated from the proteins and other metabolites present. Then, by adding a high concentration of salts to the mobile phase, it is possible to elude the DNA, which if necessary is precipitated with alcohol for future applications. Otherwise, it can be used directly dissolved in the buffer with salts in which it is eluted. (Almeida et al., 2019)
This method has the advantage of avoiding the use of toxic organic solvents and is simpler than those that require the precipitation of other components for their separation from DNA.
We hope this blog has helped you know some most common DNA extraction methods and choose the right one. However, the use of Proteinase K is an effective method in the scientific world. And to get the molecular biology grade Proteinase K, and other related products like 1000ml Erlenmeyer Flask and pipette tips, you can contact MBP Inc.