Name: 25-OH Vitamin D3 (Highly Pure)
Brand: Molecular Depot
SKU: B2010607
Price: 395.00 USD
Availability: InStock

<h2>What are research-grade vitamins?</h2>
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Research-grade vitamins are individual purified vitamin compounds, both fat-soluble (A, D, E, K) and water-soluble (B1/thiamine, B2/riboflavin, B3/niacin, B5/pantothenic acid, B6/pyridoxine, B7/biotin, B9/folic acid, B12/cobalamin, and C/ascorbic acid), supplied at cell culture grade or analytical grade purity for use as cell culture media components, enzyme cofactor studies, metabolic research, or calibration standards. Unlike commercial multivitamin preparations, research-grade individual vitamins have documented purity, lot-specific certificates of analysis, and defined chemical forms that allow precise supplementation without unknown compositional variation.
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<h2 dir="ltr">What you will find:</h2>
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Culture-Grade Vitamin Complexes: Ultra-pure essential coenzymes optimized for maintaining healthy cell line growth and metabolic activity.
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Water-Soluble Coenzymes: Stable B-complex and Vitamin C formulations prepared for metabolic pathway mapping and antioxidant studies.
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Fat-Soluble Vitamin Analogs: High-purity Vitamin A, D, E, and K derivatives calibrated for cell differentiation and lipid signaling research.
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Growth Factor Suppressors: Specialized vitamin configurations designed to study nutritional deficiencies and metabolic inhibition mechanisms.
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<h2>How to choose and use research-grade vitamins</h2>
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Confirm the specific chemical form of the vitamin you need
Many vitamins exist as multiple chemical forms with different biological activities and solubilities: vitamin B6 is available as pyridoxine hydrochloride, pyridoxal-5-phosphate, or pyridoxamine, each with different roles as enzyme cofactors and different stabilities; and vitamin D is available as cholecalciferol (D3) or ergocalciferol (D2). Specifying the exact form and salt, if applicable, ensures you source what your protocol or media formulation requires.
Handle fat-soluble vitamins under light-protected, inert-atmosphere conditions
Fat-soluble vitamins A, D, E, and K are susceptible to oxidative degradation from light and oxygen exposure, and should be stored in light-protected containers, ideally under nitrogen or argon atmosphere, at the recommended low temperature to maintain potency over their shelf life.
Prepare aqueous solutions of fat-soluble vitamins using appropriate solubilization
Fat-soluble vitamins require a co-solvent, carrier protein, or emulsifier for preparation as aqueous working solutions, since they are poorly soluble in water alone; ethanol or DMSO at minimal volume, or BSA complexation, are common solubilization approaches for preparing cell culture-compatible working solutions.
Confirm vitamin stability in your storage conditions before preparing large stocks
Water-soluble vitamins like ascorbic acid (vitamin C) and riboflavin are particularly susceptible to oxidation or photodegradation and may have short working-solution half-lives at room temperature, making fresh preparation or careful storage in aliquots at low temperature preferable to maintaining large working stocks.
Use vitamins with lot-specific CoA when serving as analytical calibration standards
When a vitamin compound is used as a quantitative analytical standard rather than just a biochemical supplement, confirmed purity from a lot-specific certificate of analysis is required for accurate calibration, since assumed purity can introduce systematic error in any quantitative assay.
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<h2>Specifications context</h2>
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Individual research-grade vitamins are supplied in characterized forms with documented purity, in contrast to complex multi-ingredient vitamin preparations whose exact composition may be less precisely defined, which matters for cell culture applications where trace impurities in vitamin supplements can affect cell viability and for analytical applications where purity directly affects calibration accuracy. As of 2026, water-soluble vitamin compounds including the B-complex vitamins and ascorbic acid continue to be among the most frequently purchased biochemical supplement reagents for defined cell culture media formulations.
<a href="https://mbpinc.net/contact-us">Contact the helpful team at MBP</a> today to request a quote for your specific research requirements.

Vitamins

Molecular Depot

Key Specifications

25-OH Vitamin D3 (Highly Pure)

25-OH Vitamin D3 (Highly Pure) by Molecular Depot is a highly pure 25-hydroxyvitamin D3, the monohydroxylated metabolite of vitamin D3. Its molecular weight is 400.64 g/mol. It is used as a calibrator and standard in vitamin D assays and in vitamin D metabolism research. Each unit contains 5 mg. MBP supplies Molecular Depot vitamin reagents to research labs in the United States, Canada and worldwide.