Name: 2-Nitrophenyl-β-D-galactopyranoside
Brand: Molecular Depot
SKU: B2015366
Price: 695.00 USD
Availability: InStock

<h2>What are research carbohydrates and derivatives?</h2>
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Research-grade carbohydrates and their derivatives cover the full structural range of sugar-based compounds: monosaccharides like glucose, fructose, mannose, and galactose that serve as cell culture carbon sources and enzyme substrates; disaccharides like sucrose, lactose, and trehalose used in biochemical assays and cryoprotection; sugar alcohols like sorbitol and mannitol with osmotic properties; amino sugars like glucosamine and N-acetylglucosamine as glycan precursors; and glycoside substrates for measuring glycosidase enzyme activity.
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<h2 dir="ltr">What you will discover:</h2>
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IPTG Expression Inducers: High-purity Isopropyl beta-D-1-thiogalactopyranoside designed for consistent activation of lac promoter-driven protein production.
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L-Arabinose Control Sugars: Enhanced metabolic activators fine-tuned for activating tightly controlled araBAD promoter gene expression systems.
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Cumate Induction Agents: Tailored chemical agents designed for the accurate adjustment of cumate-switchable mammalian and bacterial systems.
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Functional Glycosides &amp; Analogs: Refined carbohydrate derivatives designed for exploring enzyme substrate specificity and interactions with cell surface receptors.
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<h2>How to choose research carbohydrates</h2>
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Use glucose or fructose as carbon sources for cell culture or microbial growth
D-Glucose is the most common carbohydrate supplement for mammalian cell culture media and microbial growth, while fructose and mannose are used in experiments specifically studying carbon source preferences, hexose transport, or sugar metabolism pathways.
Choose trehalose or sucrose for cryoprotection and protein stabilization
Trehalose is widely used as a non-reducing disaccharide stabilizer for proteins, liposomes, and cells during freeze-drying or cryogenic storage, and sucrose is used similarly as an osmotic stabilizer. Neither trehalose nor sucrose participate in the Maillard browning reaction, making them superior stabilizers compared with reducing sugars.
Use sugar alcohols for osmolarity adjustment and osmoprotection
Sugar alcohols such as sorbitol and mannitol are commonly used to create defined osmotic conditions in cell biology experiments, and as osmotic controls in studies of cell volume regulation, without being metabolized by most mammalian cell types.
Choose N-acetylglucosamine or N-acetylgalactosamine for glycobiology applications
These amino sugar derivatives serve as biosynthetic precursors and enzyme substrates for studying O-GlcNAc modification, mucin-type glycosylation, and chitin biosynthesis, and are substrates for hexosaminidase activity assays.
Use chromogenic or fluorescent glycoside substrates for enzyme activity measurement
Synthetic glycoside substrates with a chromogenic aglycone, such as p-nitrophenyl glycosides, or fluorescent aglycone, such as 4-methylumbelliferyl glycosides, release a detectable product upon hydrolysis by the corresponding glycosidase enzyme, enabling quantitative enzyme activity measurements.
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<h2>Specifications context</h2>
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Trehalose's efficacy as a cryoprotectant and protein stabilizer is attributed to its exceptionally high glass transition temperature compared with other disaccharides and its ability to form a glassy matrix that slows molecular motion and prevents denaturation during drying or freezing. As of 2026, trehalose continues to be widely used in lyophilization and spray-drying formulations for protein and vaccine stability, driving demand for research-grade trehalose in pharmaceutical and biological research settings.
<a href="https://mbpinc.net/contact-us">Reach out to the friendly team at MBP</a> now to obtain a quote tailored to your particular research needs.

Carbohydrates & Derivatives

Molecular Depot

Key Specifications

2-Nitrophenyl-β-D-galactopyranoside

2-Nitrophenyl-β-D-galactopyranoside by Molecular Depot is a 2-nitrophenyl-β-D-galactopyranoside, known as ONPG, the chromogenic substrate of β-galactosidase. β-Galactosidase releases 2-nitrophenol, which is yellow in alkali, so the reaction is followed by absorbance at 420 nm without any further step. It is used as the substrate in colorimetric β-galactosidase assays, in lacZ reporter gene quantification and in the classical Miller assay of bacterial gene expression. Each unit contains 5 g. MBP supplies Molecular Depot carbohydrate reagents to research labs in the United States, Canada and worldwide.