The D41 Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead-based technology. This straightforward procedure is compatible with either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.
Order D41 Zyppy-96 Plasmid MagPrep Kit in USA and Canada from MBP Inc.
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Equipment | Magnetic Stand, Heating Element, or Liquid Handler |
| Processing Time | 60 min |
| Purity | Typical Abs 260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed. |
Q1: What is the composition of the Zyppy Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q2: Can the purified plasmid be transfected into eukaryotic cell lines?
Yes, all our plasmid preps have an endo-wash step to remove endotoxin very effectively and produce transfection ready high-quality plasmid DNA.
Q3: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Q4: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q5: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
Q6: I accidently left my Neutralization Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
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