The D410 Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.
Order D410 Zyppy-96™ Plasmid Kit in USA or in Canada from MBP Inc. and get the best prices from the market.
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Equipment | Centrifuge with micro plate carriers |
| Processing Time | 45 min |
| Purity | Typical Abs 260/280 ≥ 1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed. |
Q1: Can E.coli grown in enriched growth media be used with this kit?
No
Q2: What is the composition of the Zyppy Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q3: Can the purified plasmid be transfected into eukaryotic cell lines?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Q5: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q6: I accidentally left my Neutralization Buffer at room temperature. Will it be okay?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4°C as soon as possible.
Q7: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
Q8: I accidently left my Neutralization/Clearing Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
Q9: Is the sediment inside the Neutralization/Clearing Buffer supposed to go into solution?
No, the sediment inside the buffer is insoluble, but it needs to be completely resuspended in the buffer prior to use for optimal performance.
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