E205 ZymoTaq qPCR PreMix contains all the reagents needed to perform quantitative PCR and other molecular downstream analysis such as high-resolution melt (HRM) analysis of DNA methylation and other general real-time PCR assays. This premix features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treated DNA. It also includes an intense dsDNA-specific fluorescent dye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (E2005, E2006, E2007) for low copy detection of DNA from humans, bacteria, or fungi.
| Activity | 5′ – 3′ DNA polymerization |
|---|---|
| Concentration | Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq DNA polymerase. |
| Equipment | Real-time PCR can be performed on any instrument that has optical scanners that do not require passive reference dye. |
| Storage | Store at ≤ -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents. |
| Unit Definition | One unit (U) enzyme of ZymoTaq DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C. |
Q1: What is the error rate of the ZymoTaq DNA polymerase?
The error rate for ZymoTaq DNA polymerase ranges from 1.1×10-4 to 8.9×10-5.
Q2: Which is the maximal amplicon size generated using the ZymoTaq DNA polymerase?
The ZymoTaq DNA polymerase can amplify up to 5 kb.
Q3: What is the benefit of the ZymoTaq DNA PCR systems?
The ZymoTaq DNA PCR system utilizes a hot-start polymerase and is optimized for bisulfite PCR and other GC rich templates, as well as low-copy number of DNA templates. It can also be used for general molecular biology applications as well (e.g. suitable for TA cloning, multiplex PCR, etc).
Q4: Does ZymoTaq DNA polymerase have a 5’ to 3’ exonuclease activity?
Yes.
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