D4204 ZymoPURE II Plasmid Gigaprep Kit

• Fast: 50 minute Gigapreps
• Highest Yield: Purify up to 10 mg of plasmid DNA directly from a spin-column.
• Ultra-Pure: EndoZero, vaccine grade, and transfection ready.

Part of the D4204 ZymoPURE Plasmid Kits collection, the D4204 ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 10 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the D4204 ZymoPURE II Plasmid Gigaprep kit can process up to 2.5 L of culture in less than 50 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with a vacuum instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl) , EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.