Highlights
- Pellet-Free: Direct lysis procedure omits cell-pelleting, resuspension steps, and large centrifuges.
- TQuick & Pure: 15 minutes from culture flask to transfection-grade plasmid DNA.
- Highest Yield: Purify up to 1.2 mg of plasmid DNA using a spin-column.
Description
The D4213 ZymoPURE – Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The D4213 ZymoPURE – Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the D4213 ZymoPURE – Express Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing. No large centrifuge! No cell pelleting! No gravity filtration! No ethanol precipitation! From culture to endotoxin-free plasmid DNA in 15 minutes!
Applicable For
Eluted DNA is well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.
Binding Capacity
1.2 mg
Culture Input
? 25 mL
Elution Volume
? 200 ?l
Endotoxin Levels
?1 EU/?g Plasmid DNA
Equipment
Microcentrifuge and vacuum/vacuum manifold
Format
Spin Column
Processing Time
50 min
Purity
Typical Abs 260/280 ?1.8 and Abs 260/230 ? 2.0
Recommended Sample Volume
? 25 ml
Size Range
Up to ~200 kb
Yield
? 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized). 1.2 mg maximum binding capacity of column.
Q1: I accidently left my ZP Express Neutralization Buffer at room temperature, is it still stable for use?
Yes, the RNase A is fairly stable at room temperature but we recommend placing it in 4 ?C as soon as possible.
Q2: Can you recover up to 1.2 mg DNA with the ZymoPURE-Express Plasmid Midiprep Kit?
The binding capacity of the column is up 1.2 mg DNA, however, typical yields from directly processing 25 ml culture of a high copy plasmid is 100 ? 200 ug total DNA.
Q3: Can you process more than 25 ml culture?
Yes, up to 50 ml bacterial culture in LB Medium can be pelleted, resuspended, and processed with the kit (see appendix in instruction manual).
Q4: What is the composition of the ZymoPURE Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
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96 / 100 Bioz Stars |
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Mal…
Nature Communications – Published 16 Feb 2019
Vector constructs were prepared using ZymoPURE plasmid midiprep kit (CatD4201, Zymo Research, CA, USA) following manufacturer?s instructions.
Molecular safeguarding of CRISPR gene drive experiments
Plasmids used for injection into eggs were purified with ZymoPure Midiprep kit (Zymo Research).
Improving cytidine and adenine base editors by expression optimization a…
Nat Biotechnol – Published 29 Nov 2018
All plasmids were assembled by either the USER cloning method as previously described or by Gibson assembly .. Plasmids for mammalian cell transfections were prepared using an endotoxin removal plasmid purification system, ZymoPURE Plasmid Midiprep (Zymo Research Corporation).. HEK293T cells (ATCC CRL-3216) were cultured in Dulbecco?s Modified Eagle?s Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS),
| Cat # | Name | Size |
|---|---|---|
| D4200-5-55 | ZymoPURE Wash 1 | 55 ml |
| D4200-6-23 | ZymoPURE Wash 2 (Concentrate) | 23 ml |
| D4200-7-12 | ZymoPURE Elution Buffer | 12 ml |
| D4213-2-260 | ZP Express Neutralization Buffer | 260 ml |
| D4213-1-210 | ZP Express Lysis Buffer | 210 ml |
| D4213-3-260 | ZP Express Binding Buffer | 260 ml |
| C1036-5 | ZymoPURE Syringe Filters | 5 Pack |
| C1037-5 | ZymoPURE Syringe Plungers | 5 Pack |
| C1042-5 | Zymo-Spin V-P Column Assembly w/ 15 ml and 50 ml Reservoir | 5 Pack |
- Catalog#: D4213
- Package Length (in Inches): 13
- Package Width (in Inches): 8.5
- Package Height (in Inches): 13
- Package Weight (in Pounds): 7.9
- Size: 25 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
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