Q1: I accidently left my ZP Express Neutralization Buffer at room temperature, is it still stable for use?
Yes, the RNase A is fairly stable at room temperature but we recommend placing it in 4 ?C as soon as possible.
Q2: Can you recover up to 1.2 mg DNA with the ZymoPURE-Express Plasmid Midiprep Kit?
The binding capacity of the column is up 1.2 mg DNA, however, typical yields from directly processing 25 ml culture of a high copy plasmid is 100 ? 200 ug total DNA.
Q3: Can you process more than 25 ml culture?
Yes, up to 50 ml bacterial culture in LB Medium can be pelleted, resuspended, and processed with the kit (see appendix in instruction manual).
Q4: What is the composition of the ZymoPURE Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
|96 / 100
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Mal…
Vector constructs were prepared using ZymoPURE plasmid midiprep kit (CatD4201, Zymo Research, CA, USA) following manufacturer?s instructions.
Molecular safeguarding of CRISPR gene drive experiments
Plasmids used for injection into eggs were purified with ZymoPure Midiprep kit (Zymo Research).
Improving cytidine and adenine base editors by expression optimization a…
All plasmids were assembled by either the USER cloning method as previously described or by Gibson assembly .. Plasmids for mammalian cell transfections were prepared using an endotoxin removal plasmid purification system, ZymoPURE Plasmid Midiprep (Zymo Research Corporation).. HEK293T cells (ATCC CRL-3216) were cultured in Dulbecco?s Modified Eagle?s Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS),
|D4200-5-55||ZymoPURE Wash 1||55 ml|
|D4200-6-23||ZymoPURE Wash 2 (Concentrate)||23 ml|
|D4200-7-12||ZymoPURE Elution Buffer||12 ml|
|D4213-2-260||ZP Express Neutralization Buffer||260 ml|
|D4213-1-210||ZP Express Lysis Buffer||210 ml|
|D4213-3-260||ZP Express Binding Buffer||260 ml|
|C1036-5||ZymoPURE Syringe Filters||5 Pack|
|C1037-5||ZymoPURE Syringe Plungers||5 Pack|
|C1042-5||Zymo-Spin V-P Column Assembly w/ 15 ml and 50 ml Reservoir||5 Pack|
- Catalog#: D4213
- Package Length (in Inches): 13
- Package Width (in Inches): 8.5
- Package Height (in Inches): 13
- Package Weight (in Pounds): 7.9
- Size: 25 Preps
- Unit Standard: Metric
- Volume Units: Milliliters