Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 – 0.3 ng for most 2 ? based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into?E. Coli, cultured, and isolated using a traditional?E. Coli?plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Q4: If I?m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes: Susceptible fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis
|99 / 100
Tracking the origin of two genetic components associated with transposab…
Plasmids were isolated from individual yeast clones using the Zymo Yeast Plasmid Miniprep kit (Zymo Research) and transformed into Escherichia coli for plasmid purification and sequence
Reprogramming a Deubiquitinase into a Transamidase
n subsequent rounds, 5 ? 106 cells were harvested and reacted with 5, 0.5, and 0.1 ? M of biotinylated Ub D77.. The reaction time was 10 min for all selection rounds except round 5, which was 5 min. At the end of rounds three and five, plasmids were isolated from the enriched clones using a Zymoprep yeast plasmid miniprep kit (Zymo Research) and electroporated into DH10 ? cells.. Individual colonies were grown, miniprepped, and sequenced using p C T ? y u h l ? r 2 to identify beneficial mutations for autoubiquitination.
A user-friendly platform for yeast two-hybrid library screening using ne…
The cDNA inserts of the prey plasmids (pDEST22-insert) were PCR-amplified using backbone-specific primers ( ) and Sanger-sequenced.. Colonies of the Y2H screening plates were dissolved and pooled in 10?15 mL of ultrapure water and plasmids were collected using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research, Irvine, CA, USA).
|D2004-1-10||Solution 1 Digestion Buffer||10 ml|
|D2004-2-10||Solution 2 Lysis Buffer||10 ml|
|D2004-3-20||Solution 3 Neutralizing Buffer||20 ml|
|D4003-2-6||DNA Wash Buffer (Concentrate)||6 ml|
|C1001-50||Collection Tubes||50 Pack|
|C1003-50||Zymo-Spin I Columns||50 Pack|
- Catalog#: D2004
- Package Length (in Inches): 7
- Package Width (in Inches): 5
- Package Height (in Inches): 4.5
- Package Weight (in Pounds): 0.3
- Size: 50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters