The R2001 ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. |
| Size Range | Total RNA ≥17 nt |
| Yield | 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
| Supplemental Info |
Q1: What is the purpose of the Zymo-Spin III-HRC Step?
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA/RNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).
Q2: What do I do if I notice precipitation during ethanol addition?
Sample overloading can cause nucleic acids to precipitate and crash out of solution, reducing yields. Use less sample input to avoid precipitation.
Q3: What do I do if there is foaming of sample during homogenization?
This is a normal occurrence as DNA/RNA Shield contains detergents. To reduce foam, centrifuge at 12,000 x g in 1 minute intervals before transferring the supernatant.
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