DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
RNA > 1 month
DNA > 2 years
DNA & RNA (<-20?C): Indefinitely
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ?l of FACS sorted cells into minimally 400 ?l of DNA/RNA Shield).
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37?C for 5 minutes and vortex.
No, tissues do not need to be homogenized.
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
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Presence of the point mutations Val1016Gly in the voltage-gated sodium c…
Parasit Vectors – Published 28 Jan 2019
The paddles with eggs were immersed in trays of water and were reared to adult stage.. Subsequently, the mosquitoes were placed in 1.5 ml conical tubes with 500 ?l of DNA/RNA Shield? (Zymo Research, Irvine, CA, USA) and stored at -80 ?C.. The genomic DNA of the mosquitoes was isolated with a ZR Viral DNA/RNA Kit? (Zymo Research) according to the manufacturer?s instructions.
A quasi-integral controller for adaptation of genetic modules to variabl…
Nat Commun – Published 21 Dec 2018
least 120,000 singlet events were collected for data analysis with the FlowJo v10 (FlowJo, LLC) and the CFlowPlus v1.0.264.14 (Becton Dickinson) softwares. . At the end of the last batch of an experiment (OD600nm ~0.05), 200 ?L E. coli cells were lysed with 1150 ?L DNA/RNA Shield 2? concentrate solution (Zymo Research R1200-125).. Total RNA was extracted with Quick-RNA Microprep Kit (Zymo Research, R1051) and quantified with the nanophotometer (Implen, P330).’
Phylogenetic analysis of West Nile Virus in Maricopa County, Arizona: Ev…
PLoS One – Published 26 Nov 2018
(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Zika virus sequencing. . Prior to transporting the samples, an equal part of DNA/RNA ShieldTM 2X Concentrate (Zymo Research) was added to each mosquito pool to stabilize the RNA and inactivate WNV.. The pools consisted of mosquitos in approximately 1.3 ml of TE buffer (Invitrogen, AM9858).
?I?m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!?
– Laura T. (USDA)
?We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4?C or -20?C for months, never saw any DNA degradation.?
– Umberto P. (University of Pavia)
?I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4?C for up to a month.?
– Maha AE. (University of Florida)
?We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.?
– Mariska B. (University of Amsterdam)