Highlights
- Accurate composition: composition cross-validated with multiple types of measurements
- Negligible impurity: guaranteed to contain < 0.01% foreign microbial DNA.
- Wide range of GC content: 15%-85%, for assessing bias caused by GC content variation
Description
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The D6305 ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% – 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes – 12%, Pseudomonas aeruginosa – 12%, Bacillus subtilis – 12%, Escherichia coli – 12%, Salmonella enterica – 12%, Lactobacillus fermentum – 12%, Enterococcus faecalis – 12%, Staphylococcus aureus – 12%, Saccharomyces cerevisiae – 2%, and Cryptococcus neoformans – 2%.
Purity
< 0.01% foreign microbial DNA
Sample Source
A mixture of genomic DNA of ten microbial strains.
Sample Storage
-20 ?C
 |
94?/ 100 Bioz Stars |
Evaluation of DESS as a storage medium for microbial community analysis
A ZymoBIOMICS Microbial Community Standard (Zymo Research Corporation, Irvine, CA, USA) composed of five Firmicutes (Gram positive) and three Proteobacteria (Gram negative) was used to precisely test DESS?s ability to preserve microbiome DNA.. Although not as diverse as T.
Cloacal and Ocular Microbiota of the Endangered Australian Northern Quol…
Microorganimsm – Published 12 Jul 2018
See supplementary methods for a detailed PCR protocol ( ).. Sequencing controls included DNA extraction process negatives, PCR negatives, and two mock community standards, the even mock community from the Human Microbiome Project ( https://www.hmpdacc.org/HMMC/ ) which is a defined mixture of DNA from of 21 bacterial and one yeast species, and the Zymobiomics microbial community DNA standard (Zymo Research, D6305, Irvine, CA, USA) which contains eight bacterial and two yeast genomes.. PCRs from each sample were pooled equally by molarity.
Evaluating Established Methods for Rumen 16S rRNA Amplicon Sequencing Wi…
Front Microbial – Published 25 Jun 2018
n order to replicate the DNA extraction protocol identically to our rumen extraction protocol, the ZymoBIOMICSTM Microbial Community Standard (Zymo Research Corp., Irvine, CA, United States) was diluted in a 2-mL microfuge tube (DNA/RNA/DNase/RNase-free; Merck KGaA, Darmstadt, Germany) with molecular grade phosphate buffered saline (PBS; Merck KGaA, Darmstadt, Germany), and the tube and its contents were snap frozen in liquid nitrogen.. The frozen tube contents were then homogenized to a fine frozen powder under liquid nitrogen using a pestle and mortar and the frozen powder was stored
- Catalog#: D6305 /D6306
- Package Length (in Inches): 1.8
- Package Width (in Inches): 0.4
- Package Height (in Inches): 0.4
- Package Weight (in Pounds): 0.1
- Size: 200ng /2000ng
- Unit Standard: Metric
- Volume Units: Milliliters
Reviews
There are no reviews yet.