Evaluation of DESS as a storage medium for microbial community analysis
A ZymoBIOMICS Microbial Community Standard (Zymo Research Corporation, Irvine, CA, USA) composed of five Firmicutes (Gram positive) and three Proteobacteria (Gram negative) was used to precisely test DESS?s ability to preserve microbiome DNA.. Although not as diverse as T.
Cloacal and Ocular Microbiota of the Endangered Australian Northern Quol…
See supplementary methods for a detailed PCR protocol ( ).. Sequencing controls included DNA extraction process negatives, PCR negatives, and two mock community standards, the even mock community from the Human Microbiome Project ( https://www.hmpdacc.org/HMMC/ ) which is a defined mixture of DNA from of 21 bacterial and one yeast species, and the Zymobiomics microbial community DNA standard (Zymo Research, D6305, Irvine, CA, USA) which contains eight bacterial and two yeast genomes.. PCRs from each sample were pooled equally by molarity.
Evaluating Established Methods for Rumen 16S rRNA Amplicon Sequencing Wi…
n order to replicate the DNA extraction protocol identically to our rumen extraction protocol, the ZymoBIOMICSTM Microbial Community Standard (Zymo Research Corp., Irvine, CA, United States) was diluted in a 2-mL microfuge tube (DNA/RNA/DNase/RNase-free; Merck KGaA, Darmstadt, Germany) with molecular grade phosphate buffered saline (PBS; Merck KGaA, Darmstadt, Germany), and the tube and its contents were snap frozen in liquid nitrogen.. The frozen tube contents were then homogenized to a fine frozen powder under liquid nitrogen using a pestle and mortar and the frozen powder was stored
- Catalog#: D6305 /D6306
- Package Length (in Inches): 1.8
- Package Width (in Inches): 0.4
- Package Height (in Inches): 0.4
- Package Weight (in Pounds): 0.1
- Size: 200ng /2000ng
- Unit Standard: Metric
- Volume Units: Milliliters