Zymo-Spin I Columns

Presence of the point mutations Val1016Gly in the voltage-gated sodium c…

Parasit Vectors – Published 28 Jan 2019

The paddles with eggs were immersed in trays of water and were reared to adult stage.. Subsequently, the mosquitoes were placed in 1.5 ml conical tubes with 500 ?l of DNA/RNA Shield? (Zymo Research, Irvine, CA, USA) and stored at -80 ?C.. The genomic DNA of the mosquitoes was isolated with a ZR Viral DNA/RNA Kit? (Zymo Research) according to the manufacturer?s instructions.

A quasi-integral controller for adaptation of genetic modules to variabl…

Nat Commun – Published 21 Dec 2018

least 120,000 singlet events were collected for data analysis with the FlowJo v10 (FlowJo, LLC) and the CFlowPlus v1.0.264.14 (Becton Dickinson) softwares. . At the end of the last batch of an experiment (OD600nm ~0.05), 200 ?L E. coli cells were lysed with 1150 ?L DNA/RNA Shield 2? concentrate solution (Zymo Research R1200-125).. Total RNA was extracted with Quick-RNA Microprep Kit (Zymo Research, R1051) and quantified with the nanophotometer (Implen, P330).’

Phylogenetic analysis of West Nile Virus in Maricopa County, Arizona: Ev…

PLoS One – Published 26 Nov 2018

(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Zika virus sequencing. . Prior to transporting the samples, an equal part of DNA/RNA ShieldTM 2X Concentrate (Zymo Research) was added to each mosquito pool to stabilize the RNA and inactivate WNV.. The pools consisted of mosquitos in approximately 1.3 ml of TE buffer (Invitrogen, AM9858).

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