Description
The D3096 Zymo-Seq UDI Primer Sets contain pre-mixed Unique Dual Index (UDI) primers that can be used to barcode any library containing TruSeq adapters. Indexes in the D3096Â Zymo-Seq UDI Primer Set (Indexes 1-12) are dispensed in 1.5 mL tubes (Cat. No. D3008), and the D3096Â Zymo-Seq UDI Primer Plate (Indexes 1-96) are dispensed in single-use foil-sealed 96-well plates (Ca. No. D3096). Indexed libraries are compatible with any Illumina sequencer. These indexes are compatible with the D3096 Zymo-Seq RiboFree Total RNA Library Kit. Order it now from MBP Inc in USA or in Canada at the best price.
Q1: Are these individual primers or pre-mixed primer sets?
Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 ?M total (2.5 ?M each primer).
Q2: What are the sequences of the indexes?
Please view the ?Zymo-Seq UDI Primer Set 1-96? spreadsheet for index sequences and adapter trimming parameters.
Q3: Will this work with Nextera adapters?
No, these index primers only work with TruSeq compatible libraries.
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