Q1: Are these individual primers or pre-mixed primer sets?
Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 ?M total (2.5 ?M each primer).
Q2: What are the sequences of the indexes?
Please view the ?Zymo-Seq UDI Primer Set 1-96? spreadsheet for index sequences and adapter trimming parameters.
Q3: Will this work with Nextera adapters?
No, these index primers only work with TruSeq compatible libraries.
- Catalog#: D3008
- Package Length (in Inches): 6
- Package Width (in Inches): 4
- Package Height (in Inches): 0.5
- Size: 20 ul / index
- Unit Standard: Metric
- Volume Units: Milliliters