The D40 Zymo ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Order it now from MBP Inc. in the USA and Canada.
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Endotoxin Levels | < 50 EU/µg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Q1: What is the difference between the ZymoPURE Miniprep Kit and the ZR Plasmid Miniprep – Classic?
The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.
Q2: Can the kit be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.
Q3: Can the purified plasmid be transfected into eukaryotic cell lines (i.e. Is it transfection grade)?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q4: What is the composition of the DNA Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q5: Can I perform the procedure using a vacuum manifold?
Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.
Q6: I ran out of Plasmid Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.
Q7: What are the endotoxin levels in plasmid DNA isolated using the ZR-Plasmid Miniprep Classic Kit?
50 Endotoxin Units/µg of plasmid DNA.
Q8: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
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