The D405 Zymo ZR DNA Sequencing Clean-Up Kits provide a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or “dye blobs” which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.
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| Elution Volume | 6 µl – 20 µl |
|---|---|
| Equipment | Microcentrifuge |
| Sample Source | Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products |
| Sample storage | Eluted DNA can be used immediately or stored at ≤ -20°C |
| Yield | Up to 5 µg DNA |
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