Name: Zebrafish Testicular Feeder Cells (ZtA6-3)
SKU: T2602
Availability: InStock

Zebrafish Testicular Feeder Cells (ZtA6-3)

QTY/SELLING UNIT

1.0 ml

Details

Specifications

Description	ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm’s tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm. ZtA6-3 is clone 3 derived from the ZtA6 zebrafish testicular cells. ZtA6-3 cells express Sox9a and WT1 markers, and the ability to undergo phagocytosis. Clone 3 may be used as a source of homogenous mRNA and protein to assess, a source of a homogenous cell line for experimental genetic manipulation, and to aid in the research of spermatogenesis in a zebrafish model.
SKU	T2602
Species	Zebrafish (D. rerio)
Species description	Albino-type (alb-1/alb-1) zebrafish (Danio rerio)
Tissue/Organ/Organ System	Reproductive
Cell Morphology	Epithelial-like, Fibroblast-like
Applications	For Research Use Only.
Cell Type	Feeder Cells
Growth Properties	Adherent
Propagation Requirements	Grow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mare’s serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), fetal bovine serum (TM999) to a final concentration of 3%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Atmosphere: air: 100%, Temperature: 28.0°C. To prepare as feeder cells: Treat with 10 µg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
Unit quantity	1×10⁶ cells/ml
QC	1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads; 2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers; 3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells.
Storage Condition	-180°C
Shipping Conditions	On Dry Ice
Disclaimer	1. For for-profit organizations and corporations, please contact quotes@abmgood.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm‘s cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.”
Caution	For Research Use Only.

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References

Kurita, K., & Sakai, N. (2004). Functionally distinctive testicular cell lines of zebrafish to support male germ cell development. Molecular reproduction and development, 67(4), 430–438. https://doi.org/10.1002/mrd.20035

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SKU: T2602 Categories: ABM Products, iPSc and Feeder Cells, Premium Cell Culture Research Kits Tag: Albino-type (alb-1/alb-1) zebrafish (Danio rerio)

Description

Description	ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm’s tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm. ZtA6-3 is clone 3 derived from the ZtA6 zebrafish testicular cells. ZtA6-3 cells express Sox9a and WT1 markers, and the ability to undergo phagocytosis. Clone 3 may be used as a source of homogenous mRNA and protein to assess, a source of a homogenous cell line for experimental genetic manipulation, and to aid in the research of spermatogenesis in a zebrafish model.
SKU	T2602
Species	Zebrafish (D. rerio)
Species description	Albino-type (alb-1/alb-1) zebrafish (Danio rerio)
Tissue/Organ/Organ System	Reproductive
Cell Morphology	Epithelial-like, Fibroblast-like
Applications	For Research Use Only.
Cell Type	Feeder Cells
Growth Properties	Adherent
Propagation Requirements	Grow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mare’s serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), fetal bovine serum (TM999) to a final concentration of 3%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Atmosphere: air: 100%, Temperature: 28.0°C. To prepare as feeder cells: Treat with 10 µg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
Unit quantity	1×10⁶ cells/ml
QC	1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads; 2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers; 3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells.
Storage Condition	-180°C
Shipping Conditions	On Dry Ice
Disclaimer	1. For for-profit organizations and corporations, please contact quotes@abmgood.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm‘s cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.”
Caution	For Research Use Only.