The R1002 Zymo YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
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Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications |
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Elution Volume | ≥ 60 µl DNase/RNase-Free Water |
Equipment | Microcentrifuge & Heat Block/Bath |
Processing Time | 30 minutes |
Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107 cells) |
Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
Size Range | ≥ 17 nt |
Type | Total RNA |
Yield | ≤ 25 µg of total RNA |
Q1: What strains is this kit compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis
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