The D2002 Zymo YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 – 20 µg of DNA with a size distribution of 35 – 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
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| Applicable For | Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 60 µl TE or DNase-free Water |
| Equipment | Microcentrifuge, heat block/bath |
| Processing Time | 1.5 hours |
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107 cells) |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA. |
| Type | Total DNA |
| Yield | ≤ 25 µg of total DNA |
Q1: What is the difference between protocol I and protocol II?
Protocol I and II are almost same, except that chloroform is used in Protocol I. Protocol I usually gives more recovery of DNA by 30-80% compared to Protocol II. Protocol II is chloroform-free. Chloroform is not provided.
Q2: What strains is this kit compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera:
Asbya,
Kloekera,
Candida,
Kluyveromyces,
Debaryomyces,
Lipomyces,
Eremothecium,
Metschikowia,
Endomyces,
Pichia,
Hansenula,
Pullularia,
Hanseniaspora,
Saccharomyces,
Saccaromycodes,
Saccharomycopsis,
Schizosaccahromyces,
Torulopsis
Q3: How can I improve my DNA yield?
Zymolyase is very efficient, but here are some tips to maximize DNA yield.
- Use fresh and early log phase cells, which are more susceptible to the lytic properties of Zymolyase.
- Using less cells as input can improve overall lysis efficiency.
- Zymolyase digestion time can also be increased to 2 – 16 hours
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