Viral RNA Buffer

Norovirus Cell Tropism is Determined by Combinatorial Action of a Viral …

Cell Host Microbe Published 11 Oct 2018

‘Plates were incubated for 60?72 hours prior to visualization of plaques with crystal violet solution (0.2% crystal violet and 20% ethanol).. As previously described , RNA was isolated from stool using a ZR-96 viral RNA kit (Zymo Research, Irvine, CA).. RNA from tissues or cells was isolated using TRI Reagent (Invitrogen) with a Direct-zol-96 RNA kit (Zymo Research, Irvine, CA) according to the manufacturer?s ‘

Viral Entry Properties Required for Fitness in Humans Are Lost through R…

mBio Published 3 Jul 2018

‘mNGS was performed as previously described ( ).. RNA was extracted from 100 ?l of thawed culture harvests and clinical samples using the Zymo Viral RNA kit (Zymo Research) and treated with Turbo DNase I (Thermo Fisher) for 30 min at 37?C ( ).. Double-stranded cDNA was made using random hexamers, SuperScript III reverse transcriptase (Thermo Fisher), and Sequenase v2.0 (Thermo Fisher) and ‘

Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Pro…

Front Public Health Published 18 May 2018

‘RNA extracted from serum samples according to methods described by Londono-Renteria et al. ( ) using DENV serotypes specific primers described elsewhere ( ). . Briefly, RNA was extracted from the participant?s serum sample using the Quick RNA-Viral kit (Zymo Research). qRT-PCR conditions on the CFX96 Touch? Real-Time PCR Detection System were: RT Step: 48?C for 5 min, and 95?C for 2 min. Amplification step: (95?C for 15 s, 60?C for 20 s) ? 40 cycles.. The living conditions of participants are based on the socioeconomic stratification for the Barrios (Neighborhoods) in the city (Cucuta) according ‘