|Description||TR146 cells were developed from squamous carcinomas of the buccal mucosa that had infiltrated a lymph node. This cell line forms colonies on plastic substratum but is incapable of forming on semisolid support of soft agar. The TR146 cell line consists of a keratin 8 & 18 cytoskeleton. This cell line can be used to assess drug sensitivity to spermatogonial stem cells (SSC) cells. TR146 has been shown to be tumorigenic in mice, rapidly producing tumors. This cell line has been used in cytotoxicity studies, transport studies with C-reactive protein, barrier function studies, and in viability and metabolic studies. Overall, this cell line is suitable for drug transport studies as it replicates normal human buccal epithelium.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||30,000 cells/cm2; Recommended split ratio 1:4 to 1:10|
|Population Doubling Time||22 – 32 hours|
|Donor Age||67 years old|
|Donor Disease||Squamous cell carcinoma|
|Donor Ethnicity||Not disclosed|
|Cell Type||Tumor Cells|
|Propagation Requirements||Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
Ham’s F12 + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) + 2 mM L-glutamine (G275), 37.0°C, 5% CO₂
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1×106 cells / 1.0 ml|
1. For for-profit organizations and corporations, please contact firstname.lastname@example.org for pricing of this item.
2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at email@example.com.
3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
5. All of abm‘s cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period.”
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- Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
- Rupniak, H. T., Rowlatt, C., Lane, E. B., Steele, J. G., Trejdosiewicz, L. K., Laskiewicz, B., Povey, S., & Hill, B. T. (1985). Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. Journal of the National Cancer Institute, 75(4), 621–635.