Name: T4 RNA Ligase 2 (dsRNA Ligase)
SKU: E011
Price: 90.00 USD
Availability: InStock

T4 RNA Ligase 2 (dsRNA Ligase)

Description	T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3’→5′ phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (Cat. No. E010) which ligates single-stranded RNA, T4 RNA Ligase 2 joins knicks on double-stranded RNA and can also ligate the 3’OH of RNA to the 5’ phosphate of DNA in a double stranded structure. T4 RNA Ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5’ end in which case a truncated version of T4 RNA Ligase 2 (Cat. No. E012) is a better enzyme because it generates less side-reaction ligation products than the full length enzyme.

Unit quantity	100 μl
Applications	Ligating nicks in double-stranded RNA Ligating the 3’OH of RNA to the 5’phosphate of DNA
Concentration	10 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 35 mM (NH4)2SO4 and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of T4 RNA Ligase 2 that ligates 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

T7 RNA Polymerase $ 65.00

SKU: E011 Categories: ABM Products, Enzymes

Description	T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3’→5′ phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 (Cat. No. E010) which ligates single-stranded RNA, T4 RNA Ligase 2 joins knicks on double-stranded RNA and can also ligate the 3’OH of RNA to the 5’ phosphate of DNA in a double stranded structure. T4 RNA Ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5’ end in which case a truncated version of T4 RNA Ligase 2 (Cat. No. E012) is a better enzyme because it generates less side-reaction ligation products than the full length enzyme.

Unit quantity	100 μl
Applications	Ligating nicks in double-stranded RNA Ligating the 3’OH of RNA to the 5’phosphate of DNA
Concentration	10 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 35 mM (NH4)2SO4 and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of T4 RNA Ligase 2 that ligates 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.