|Description||T-CDS17 cells were derived from tumor cells of a male human with dedifferentiated chondrosarcoma. This cell line is able to proliferate in subcutaneous and orthopedic animal models. T-CDC17 cells are stem cell related, invasive, and constitutes all the mutations present in the chondrosarcoma tumor cells with two additional mutations in TP53 and amplification of Mouse Double Minute 2 homolog (MDM2). This cell line may be used for further investigation of cancer stem cells.|
|Species||Human (H. sapiens)|
|Cell Morphology||Mesenchymal like|
|Applications||For Research Use Only|
|Seeding Density||10000 – 20000 cells/cm2|
|Population Doubling Time||48 hours|
|Donor Age||49 years old|
|Donor Disease||Dedifferentiated chondrosarcoma|
|Cell Type||Tumor Cells|
|Expression Profile||IDH2 mutant (p.R172G); TP53 mutant (p.P72R; P.S215R); MDM2 amplification|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1×106 cells / 1.0 ml|
|QC||1) Sanger sequencing; 2) qPCR|
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Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
Rey, V., Menendez, S. T., Estupiñan, O., Rodriguez, A., Santos, L., Tornin, J., … Rodriguez, R. (2019). New Chondrosarcoma Cell Lines with Preserved Stem Cell Properties to Study the Genomic Drift During In Vitro/In Vivo Growth. Journal of Clinical Medicine, 8(4), 455. doi: 10.3390/jcm8040455