|Description||T-CDS17#4 cells were derived from tumor cells of a male human with dedifferentiated chondrosarcoma. This cell line is able to proliferate in subcutaneous and orthopedic animal models. T-CDC17 cells are stem cell related, invasive, and constitutes all the mutations present in the chondrosarcoma tumor cells with two additional mutations in TP53 and amplification of Mouse Double Minute 2 homolog (MDM2). This cell line may be used for further investigation of cancer stem cells.|
|Species||Human (H. sapiens)|
|Cell Morphology||Mesenchymal like|
|Applications||For Research Use Only|
|Seeding Density||10000 – 20000 cells/cm2|
|Population Doubling Time||30 hours|
|Donor Age||49 years old|
|Donor Disease||Dedifferentiated chondrosarcoma|
|Cell Type||Tumor Cells|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1×106 cells / 1.0 ml|
|QC||1) Sanger sequencing; 2) qPCR|
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3. All test parameters provided in the CoA are conducted using abm‘s standardized culture system and procedures. The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
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Hitora, T., Yamamoto, T., Akisue, T., Marui, T., Nakatani, T., Kawamoto, T., … & Kurosaka, M. (2005). Establishment and characterization of a KIT‐positive and stem cell factor‐producing cell line, KTHOS, derived from human osteosarcoma. Pathology international, 55(2), 41-47.
Rey, V., Menendez, S. T., Estupiñan, O., Rodriguez, A., Santos, L., Tornin, J., … Rodriguez, R. (2019). New Chondrosarcoma Cell Lines with Preserved Stem Cell Properties to Study the Genomic Drift During In Vitro/In Vivo Growth. Journal of Clinical Medicine, 8(4), 455. doi: 10.3390/jcm8040455