Specifications
| SKU | E075 |
|---|---|
| Unit quantity | 2500 U (250 μl) |
| Applications | Restriction enzyme digestion of double-stranded DNA. |
| Components | SpeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). |
| Concentration | 10000 U/ml |
| Expression System | An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). The SpeI enzyme recognizes and cuts the sequence A^CTAGT. |
| Inactivation Protocol | 80°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 200 µg/ml BSA, 250 mM NaCl, 0.15% Triton®X-100, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
