|The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
The saCas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type saCas9. Such a saCas9 protein retains its ability to bind to genomic DNA through sgRNA:genomic DNA base pairing, however, the saCas9 Null Mutant does not induce cleavage. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression.
The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).
|Cas Protein Marker
|saCas9d, Dead saCas9, saCas9 Double Mutant, Nuclease-deficient saCas9, CRISPR-associated endonuclease Cas9 from Staphylococcus aureus
|6.5µg (50pmol/ 50µL)
|1000 nM, 130 µg/ml
|Enzyme supplied with 10X Reaction Buffer
|<1.0 EU/μg of recombinant protein as determined by the LAL method.
|10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
|Store all components at -20°C.
|This product is distributed for laboratory research only. Caution: Not for diagnostic use.