R1201
Highlights
Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.
Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.
High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.
Description
The R1201 Zymo Quick-RNA Whole Blood kit utilizes DNA/RNA Shield?, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ? 6 ?l of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.
Equipment
Microcentrifuge, vortex
Purity
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene? Blood RNA Tube, etc.) and nucleated blood.
Size Range
Total RNA ? 17 nt
Yield
10 ?g RNA (binding capacity), ?6 ?l (elution volume)
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99 / 100 Bioz Stars |
RNA was extracted from canine blood using the Quick-RNA Whole Blood kit and used for PCR amplification for cloning canine TREM-1. TREM-1 expression was shown to be an amplifier or pro-inflammatory responses and has potential to be a biomarker for infection and pneumonia.
Li, J et al. Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils. Developmental and Comparative Immunology. 2011.
The Quick-RNA Whole Blood kit was used to purify 200 ul human blood and RNA was used to generate cDNA for cloning. Data suggests that the SNPs of aminopeptidase ERAP1 can encode for different normal, hypo-, or hyper- functional activities.
HCV RNA was extracted from sera of human patients using the Quick-RNA Whole Blood kit and used for amplification and Roche 454 pyrosequencing. Results reveal that more genotype 1a isolates were associated with resistance to protease inhibitors.
The Quick-RNA Whole Blood kit was used with peripheral blood stored at 4?C and extracted total RNA was used for real-time PCR gene expression analysis. Results suggest that upregulation of mRNA levels of ATP13A1, PARK, and ZNF746 can be observed in both untreated patients and preclinical stages of Parkinson?s disease.
Researchers used the Quick-RNA Whole Blood kit to extract total RNA from blood cell pellets derived from bovine blood stored in anticoagulant ACD. High RNA integrity (RIN >7) was obtained and used for microarray hybridization.
The Quick-RNA Whole Blood kit was used to extract RNA from human blood stored in DNA/RNA Shield Blood Collection Tubes and then Minichromosome maintenance complex component (MCM) 8 and 9 expression were assessed by qPCR. Greater MCM8 expression occurred in the follicular phase of the menstrual cycle.
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