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RNA was extracted from canine blood using the Quick-RNA Whole Blood kit and used for PCR amplification for cloning canine TREM-1. TREM-1 expression was shown to be an amplifier or pro-inflammatory responses and has potential to be a biomarker for infection and pneumonia.
Li, J et al. Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils. Developmental and Comparative Immunology. 2011.
The Quick-RNA Whole Blood kit was used to purify 200 ul human blood and RNA was used to generate cDNA for cloning. Data suggests that the SNPs of aminopeptidase ERAP1 can encode for different normal, hypo-, or hyper- functional activities.
HCV RNA was extracted from sera of human patients using the Quick-RNA Whole Blood kit and used for amplification and Roche 454 pyrosequencing. Results reveal that more genotype 1a isolates were associated with resistance to protease inhibitors.
Margeridon-Thermet, S et al. Similar Prevalence of Low-Abundance Drug-Resistant Variants in Treatment-Na?ve Patients with Genotype 1a and 1b Hepatitis C Virus Infections as Determined by Ultradeep Pyrosequencing. PLoS ONE. 2014
The Quick-RNA Whole Blood kit was used with peripheral blood stored at 4?C and extracted total RNA was used for real-time PCR gene expression analysis. Results suggest that upregulation of mRNA levels of ATP13A1, PARK, and ZNF746 can be observed in both untreated patients and preclinical stages of Parkinson?s disease.
Researchers used the Quick-RNA Whole Blood kit to extract total RNA from blood cell pellets derived from bovine blood stored in anticoagulant ACD. High RNA integrity (RIN >7) was obtained and used for microarray hybridization.
The Quick-RNA Whole Blood kit was used to extract RNA from human blood stored in DNA/RNA Shield Blood Collection Tubes and then Minichromosome maintenance complex component (MCM) 8 and 9 expression were assessed by qPCR. Greater MCM8 expression occurred in the follicular phase of the menstrual cycle.
Dondik, Y et al. Minichromosome maintenance complex component 8 and 9 gene expression in the menstrual cycle and unexplained primary ovarian insufficiency. Journal of Assisted Reproduction and Genetics. 2019.