The R2024 Zymo Quick-RNA Plant Miniprep Kit, which is now available in the USA and Canada at MBP Inc. provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the Quick-RNA Plant Miniprep features a specially formulated RNA Lysis Buffer. The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin III-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.
| Equipment | Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional) |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Leaves, stems, buds, flowers, fruit, seeds, etc. |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 50 µg RNA (binding capacity), ≥25 µl (elution volume) |
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